Alignments for a candidate for N515DRAFT_2924 in Pedobacter sp. GW460-11-11-14-LB5

GapMind for catabolism of small carbon sources

Alignments for a candidate for N515DRAFT_2924 in Pedobacter sp. GW460-11-11-14-LB5

Align Basic amino acid/polyamine antiporter, APA family (characterized, see rationale)
to candidate CA265_RS15000 CA265_RS15000 amino acid permease

Query= uniprot:A0A1I1Y8J0
         (492 letters)



>FitnessBrowser__Pedo557:CA265_RS15000
          Length = 491

 Score =  395 bits (1015), Expect = e-114
 Identities = 212/473 (44%), Positives = 295/473 (62%), Gaps = 20/473 (4%)

Query: 29  LKRALTARHLVLLGIGAIIGAGIFVITGQAAAEHAGPAIVLSFVFAGIACALAALCYAEF 88
           LKR L+A  LV LG+GAIIGAG+FV T  AAA++AGP++ + F+ A I CALA LCYAE 
Sbjct: 23  LKRTLSAGALVALGVGAIIGAGLFVRTAAAAAQNAGPSVTIGFIIAAIGCALAGLCYAEL 82

Query: 89  AAMLPVSGSAYSYSYATLGEYVAWFVGWSLVLEYLFTVATVAAGWSGYFNKLLALISGWI 148
           ++ +P+SGSAY+Y+YAT+GE +AW +GW LVLEY    ATV   WS Y NKLL  +    
Sbjct: 83  SSSIPISGSAYTYTYATMGELMAWVIGWDLVLEYAVGAATVGIAWSEYLNKLLVEVL--- 139

Query: 149 GHDVSLPQTLAAAPF-TVVDGHIQATGMFINLPAVAIIAAITGLCYVGITQSAFVNSIIV 207
            H   +P     +PF +  DG +      +NLPA+ I+  ++ L   G ++SAFVN +IV
Sbjct: 140 -HTSPIPYEWCHSPFQSHPDGTVNG---IMNLPALFIVGLLSLLLIKGTSESAFVNGLIV 195

Query: 208 AIKVTVILLFIAFATKYINPDNWHPFIPASE--------GASKYGWAGVGRAAAIVFFSY 259
             KV +++L I     +IN  N HP+IPA+           S  G+ GV  AA  VFF++
Sbjct: 196 ITKVGIVILIIVLGWGFINESNHHPYIPAATTYVDHAGISHSFGGFWGVIGAAGTVFFAF 255

Query: 260 IGFDAVSTAAGEAKNPQRDMPIGIIGSLILCTILYIIVAGILTGIAD---FRLLGTPEPV 316
           IGFDAVSTAA E KNP+  MPIGI+GSL +CT+LYI+ A +LTGIA    FR  G    V
Sbjct: 256 IGFDAVSTAAQETKNPKTAMPIGILGSLAVCTVLYILFAHVLTGIAPVEFFRTKGGEASV 315

Query: 317 STALDNY-PSLHWLQIIVVIGAVTGLSSVMLVMLMGQPRIFYSMARDGLIPAVFGRIHQK 375
             A+  Y     WL  +V +  + G SSV+LVML+GQ R+FYSM++DGL+P +F  +H K
Sbjct: 316 VAAISEYMTGYSWLSKLVTVAILAGFSSVILVMLLGQSRVFYSMSKDGLLPKMFSDLHPK 375

Query: 376 FRTPHVGTVVVGVLAAALGGLFNIGVLGEMVAMGTLLAFATVCIGVLVLRYTRPELPRAF 435
           F+TP+   +V+ ++           V+G+M ++GTL AF  VC+ V++LR T P+LPR F
Sbjct: 376 FKTPYKANLVILIIVGLFAAFIPGDVVGDMTSIGTLFAFMLVCVAVIILRKTDPDLPRQF 435

Query: 436 RVPVPWIVCPLGALACMALFLQSFLEHWRWMLAWIAIGQAIYFLYGYSHSKLR 488
           + P   ++  LG +AC  + L     +W  +  W+A+G  IYF Y   +S L+
Sbjct: 436 KTPWVPLIPILGVVACGLMILGLGWTNWLRLFGWLALGFIIYFGYSKKNSHLK 488


Lambda     K      H
   0.328    0.141    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 764
Number of extensions: 42
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 492
Length of database: 491
Length adjustment: 34
Effective length of query: 458
Effective length of database: 457
Effective search space:   209306
Effective search space used:   209306
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources