GapMind for catabolism of small carbon sources


Alignments for a candidate for gcvH in Pedobacter sp. GW460-11-11-14-LB5

Align Glycine cleavage system H protein; Octanoyl/lipoyl carrier protein (characterized)
to candidate CA265_RS18980 CA265_RS18980 glycine cleavage system protein H

Query= SwissProt::P64213
         (126 letters)

          Length = 126

 Score =  157 bits (397), Expect = 5e-44
 Identities = 71/125 (56%), Positives = 98/125 (78%)

           M  P+ELKY+K+HEWVKVEGN A IG+T++AQ ELGDIV+V++     E+ + + FG+VE

           +VKTVS+LY P++G V+EVN EL D+PE VN  PY K WMVKV ++D +++E LL+AE Y

Query: 121 SEMIG 125
Sbjct: 121 QELVG 125

Lambda     K      H
   0.305    0.127    0.342 

Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 78
Number of extensions: 1
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 126
Length of database: 126
Length adjustment: 14
Effective length of query: 112
Effective length of database: 112
Effective search space:    12544
Effective search space used:    12544
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.0 bits)
S2: 41 (20.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory