Align periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13) (characterized)
to candidate CA265_RS16770 CA265_RS16770 GMC family oxidoreductase
Query= reanno::Cola:Echvi_1847 (559 letters) >FitnessBrowser__Pedo557:CA265_RS16770 Length = 578 Score = 243 bits (619), Expect = 2e-68 Identities = 195/589 (33%), Positives = 277/589 (47%), Gaps = 58/589 (9%) Query: 6 YDAIVVGSGISGGWAAKELTEKGLKVLLLERGQNVEHVKDYKNATLPPWEIPHRGRRTTE 65 YDAI+VGSG GG AA L G KVLLLE GQN + D P WE P RG TT Sbjct: 13 YDAIIVGSGAGGGMAAYVLAHAGQKVLLLEAGQNFDPRLDSHQLKWP-WESPRRGASTT- 70 Query: 66 MVENHPNLKRDYVLNELVLDWWAHEDTSPYVEEKP--FTWFRGYQVGGRSLLWGRQSYRW 123 R + + W E PY ++ F WFR +GGR+ WGR S R Sbjct: 71 ---------RPFGDFDASYGGWELEG-EPYTQKNKSEFEWFRSRMLGGRTNHWGRISLRM 120 Query: 124 SDLDFEANLKEGIAVDWPIRYKDIAPWYDYVEKFAGIAGNRDGLDVLPDGEFMPPFAMNC 183 DF+ K+G+ WPI Y D+ P+YD V++ GI G +GL+ PDG FM P Sbjct: 121 GPEDFKP--KDGLTDAWPITYADVKPFYDKVDRMIGIYGTAEGLENEPDGIFMKPPKPRL 178 Query: 184 VEKDVKERIAKHFEGKRHLINSRVANITEPLPG---RPGCQARNKCWLGCPFGGYFSTQA 240 E +K+ K +I R + +TE LP R C +C C G FS+ + Sbjct: 179 NELFIKKGAEK---AGVKVITGRGSVLTEALPNNNDRAPCFYCGQCGRSCKVYGDFSSSS 235 Query: 241 STLPAAMATGNLTLRPYSIVHRVLYDKDTKKATGVEIVDAETMETIEYKSKIVFLCASAL 300 + A+ TGNLT+ ++V V+ DKD A GV V+ + ++ + K+V L ASA Sbjct: 236 CLVNPAVKTGNLTVITDAMVREVITDKDGS-AKGVSYVNRKDLQEYQVNGKLVILGASAC 294 Query: 301 NSAHVLMRSATDIWPEGLGSSSGELGHNVMDHHFRLGASGTVEGY-----DDKYYFGRRP 355 SA +L+ S + P GL +SSG +G + H GAS V G+ D K Y Sbjct: 295 ESARILLNSKSTSHPNGLANSSGVVGKYL---HDSTGAS--VSGFLPQLMDRKRYNEDGL 349 Query: 356 GGIYIPRYRNVGDDKRDYVRGFG--YQGGASRS--GWGRDVAEMNIGGPMKEA-LTEPGP 410 G ++I + + K ++ RG+ Y GG G+G VA+MN P ++ + E G Sbjct: 350 GSVHIYSPWWLDNRKLNFPRGYHIEYWGGMGMPAYGFGGGVAQMNGMVPGRDGKMKEAGG 409 Query: 411 WS--------------MGMMAFGEILPYHENTIKISKDVKDKWGMYALVMNAEIKDNEQK 456 + +GM G + +N +I + DK+G+ L N + +E Sbjct: 410 YGKSLKDDYRRFYGTGVGMAGRGTAIAREDNYCEIDPNTVDKYGIPVLRFNYKWAKDEIL 469 Query: 457 MRKDMMNDAAEMLEAAGV---KNIHTYDSGYTF---GQGIHEMGTARMGRDPKTSVLNEN 510 K M +++ G I D+ Y G+ IHE+GT RMG DPK S LN+ Sbjct: 470 QAKHMQETFLSIMKEMGAVVTSEIQGADTNYGLLNPGKIIHEVGTIRMGDDPKKSALNKY 529 Query: 511 NQVWDAKNVFVTDGAAMTSAAAVNPSLTYMALTARAADFAVKELKKGNL 559 Q D KN+FV D N + T +AL+ R A++ + + KK N+ Sbjct: 530 CQAHDCKNLFVVDAGPFVQQGDKNATWTILALSMRTAEYILAQKKKQNI 578 Lambda K H 0.317 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 855 Number of extensions: 59 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 559 Length of database: 578 Length adjustment: 36 Effective length of query: 523 Effective length of database: 542 Effective search space: 283466 Effective search space used: 283466 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory