Align Methylmalonyl-CoA epimerase; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 (characterized)
to candidate CA265_RS10815 CA265_RS10815 methylmalonyl-CoA epimerase
Query= SwissProt::O58010 (136 letters) >FitnessBrowser__Pedo557:CA265_RS10815 Length = 135 Score = 126 bits (317), Expect = 1e-34 Identities = 60/128 (46%), Positives = 89/128 (69%), Gaps = 1/128 (0%) Query: 7 RIDHVGIAVKNLEEAIKIWEGL-GFKVEEIEEVPDQKVKVAVIKVGENRIELLEATTEDS 65 +I+H+GIAVK+L +I I++ L + E+V + V A K GEN++ELL+AT DS Sbjct: 3 KIEHIGIAVKDLNSSIAIYQKLLNTDCYKTEQVASEFVNTAFFKTGENKVELLQATAPDS 62 Query: 66 PIAKFIEKRGEGIHHLAIRVENIESKLEELKQKGYKLIDEKPRVGAGGAKIAFIHPKSVT 125 IAKFIEK+GEGIHH+A V++I +++E L Q+G+ L+ E P+ GA + F+HPK Sbjct: 63 AIAKFIEKKGEGIHHIAFLVDDILAEMERLHQEGFVLLSESPKKGADDKMVCFVHPKDTN 122 Query: 126 GVLLELCE 133 GVL+E+C+ Sbjct: 123 GVLIEICQ 130 Lambda K H 0.317 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 87 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 136 Length of database: 135 Length adjustment: 15 Effective length of query: 121 Effective length of database: 120 Effective search space: 14520 Effective search space used: 14520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 42 (20.8 bits)
Align candidate CA265_RS10815 CA265_RS10815 (methylmalonyl-CoA epimerase)
to HMM TIGR03081 (mce: methylmalonyl-CoA epimerase (EC 5.1.99.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03081.hmm # target sequence database: /tmp/gapView.31005.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03081 [M=129] Accession: TIGR03081 Description: metmalonyl_epim: methylmalonyl-CoA epimerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.4e-45 139.9 0.5 3.8e-45 139.7 0.5 1.0 1 lcl|FitnessBrowser__Pedo557:CA265_RS10815 CA265_RS10815 methylmalonyl-CoA Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Pedo557:CA265_RS10815 CA265_RS10815 methylmalonyl-CoA epimerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 139.7 0.5 3.8e-45 3.8e-45 1 129 [] 3 130 .. 3 130 .. 0.99 Alignments for each domain: == domain 1 score: 139.7 bits; conditional E-value: 3.8e-45 TIGR03081 1 kldhvaiavkdleeaaklyrdvlGakvseeeelpeqgvkvvflelgetklellepleedspiakflek 68 k++h++iavkdl++ +++y+++l ++ ++e+++ + v+++f++ ge+k+ell++++ ds+iakf+ek lcl|FitnessBrowser__Pedo557:CA265_RS10815 3 KIEHIGIAVKDLNSSIAIYQKLLNTDCYKTEQVASEFVNTAFFKTGENKVELLQATAPDSAIAKFIEK 70 79****************************************************************** PP TIGR03081 69 kkgeGlhhialevddieaaletlkekgvrlldeepriGahGkkvaFlhPkdtgGvLielee 129 k geG+hhia+ vddi a +e l+++g ll+e+p+ Ga k v F+hPkdt+GvLie+++ lcl|FitnessBrowser__Pedo557:CA265_RS10815 71 K-GEGIHHIAFLVDDILAEMERLHQEGFVLLSESPKKGADDKMVCFVHPKDTNGVLIEICQ 130 *.*********************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (129 nodes) Target sequences: 1 (135 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 5.87 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory