Align D-xylulose reductase (EC 1.1.1.9) (characterized)
to candidate CA265_RS08355 CA265_RS08355 short-chain dehydrogenase
Query= BRENDA::Q8GR61 (262 letters) >FitnessBrowser__Pedo557:CA265_RS08355 Length = 254 Score = 119 bits (299), Expect = 5e-32 Identities = 79/255 (30%), Positives = 126/255 (49%), Gaps = 13/255 (5%) Query: 8 KVCLVTGAGGNIGLATALRLAEEGTAIALLDMNREALEKAEASVREKGVEARSYVCDVTS 67 K +VTG G IG A A LA++G + ++++ E + ++ G A SY CDV+ Sbjct: 7 KKAVVTGGGSGIGKAIATILAKQGAEVHIIELGTEHAQDTLDEIKTNGGVAFSYGCDVSD 66 Query: 68 EEAVIGTVDSVVRDFGKIDFLFNNAGYQGAFAPVQDYPSDDFARVLTINVTGAFHVLKAV 127 +AV +V + G I+ L NNAG DF RV+ +NV G ++ L A Sbjct: 67 HQAVA----AVFNEIGNINILINNAGI-AHIGKADTTDEADFDRVMRVNVKGVYNCLHAA 121 Query: 128 SRQMITQNYGRIVNTASMAGVKGPPNMAAYGTSKGAIIALTETAALDLAPYNIRVNAISP 187 Q+ G I+N AS+A + G P+ Y +KGA+ A+T + A D NIR N+ISP Sbjct: 122 IPQIRLSGGGVIINMASIAALIGLPDRFVYSAAKGAVKAITMSVAKDYIGENIRCNSISP 181 Query: 188 GYMGPGFMWERQVELQAKVGSQYFSTDPKVVAQQMIGSVPMRRYGDINEIPGVVAFLLGD 247 + F + + Y P++ +++ + P+ R E+ + +L D Sbjct: 182 ARVHTPF-------VDGFLQKNYPDNIPEMF-EKLSKTQPIGRMAKPEEVGALALYLCSD 233 Query: 248 DSSFMTGVNLPIAGG 262 ++SF+TG + PI GG Sbjct: 234 EASFITGCDYPIDGG 248 Lambda K H 0.318 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 175 Number of extensions: 6 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 262 Length of database: 254 Length adjustment: 24 Effective length of query: 238 Effective length of database: 230 Effective search space: 54740 Effective search space used: 54740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory