Alignments for a candidate for Echvi_1871 in Pedobacter sp. GW460-11-11-14-LB5

GapMind for catabolism of small carbon sources

Alignments for a candidate for Echvi_1871 in Pedobacter sp. GW460-11-11-14-LB5

Align SSS sodium solute transporter (characterized, see rationale)
to candidate CA265_RS06130 CA265_RS06130 sodium transporter

Query= uniprot:L0FZF3
         (547 letters)



>FitnessBrowser__Pedo557:CA265_RS06130
          Length = 558

 Score =  574 bits (1479), Expect = e-168
 Identities = 306/572 (53%), Positives = 400/572 (69%), Gaps = 48/572 (8%)

Query: 4   NTLDLVVFVAYCLLIITMGIVVSREKKGHVKDSKDYFLASKALPWWAVGASLIASNISAE 63
           +T D +VF  Y +++   G+ +  +KK     SKDYFLA  +L WWA+GASLIASNISAE
Sbjct: 7   DTKDYIVFAIYFVIVAAYGLYIYNKKKSESTGSKDYFLAEGSLTWWAIGASLIASNISAE 66

Query: 64  QFIGMSGSGFALGLAISTYEWMAAATLLVVAIFFLPIYLKEGIYTMPQFLNRRYDGRVRT 123
           QFIGMSGSGF +GLAI+TYEWM AATL+VVA+FF+P+YLK  I TMPQFL++RY+G V  
Sbjct: 67  QFIGMSGSGFKMGLAIATYEWMGAATLVVVAVFFIPVYLKNKIATMPQFLHQRYNGTVAM 126

Query: 124 VMAIFWLLIYVFVNLTSVLYLGALSLETIMGVPLTYGIIGLALFAMVYSIYGGLKAVAWT 183
           +MA+FWLL+YV VNLTS+LYLGAL++ +I G  L++ +  +A FA++ ++ GG+K + +T
Sbjct: 127 IMAVFWLLLYVVVNLTSILYLGALAVSSISGFDLSFCMYAIAGFAIIITL-GGMKVIGYT 185

Query: 184 DVVQVVFLVAGGLATTYLALSLV----GDGDVWEGIGILRKAAPSHFSMIIEKGEMMIPD 239
           DV+QV FL+ GGLATTYLAL+LV    G   ++EG  ++   A  HF MI++        
Sbjct: 186 DVIQVFFLILGGLATTYLALNLVSTHYGTTGIFEGYSLMTSKASEHFHMILKP------- 238

Query: 240 GSGGSRDAYLDLPGLSVLIGGMWIVNLNYWGCNQYITQRALAAKSLGEAQTGMVFAGFLK 299
                 + Y+DLPGLSVL+GGMWIVNLNYWGCNQYITQRAL A +L  A+ G++FA FLK
Sbjct: 239 ----ENENYIDLPGLSVLVGGMWIVNLNYWGCNQYITQRALGA-NLETARGGILFAAFLK 293

Query: 300 LLMPLIVVIPGIAAYVIVQKGADASFIESMTDPVTGLAKSDRAYPTLLHLLPPGLKGLAF 359
           LLMP+IVV+PGIAAYV+ + GA  S  E + D   G    DRAYP LL+LLP GLKGL+F
Sbjct: 294 LLMPIIVVLPGIAAYVLFKDGAFQS--EMLQD---GAVNPDRAYPVLLNLLPAGLKGLSF 348

Query: 360 AALTAAIVSSLASMANSTSTIFTIDIYKEFFNKNVSEGKQVTIGRITAVVAFIIAAIVAP 419
           AALTAA+V+SLA  ANS +TIFT+DIYK+    + +E   VT G+I+ +VA I+  ++AP
Sbjct: 349 AALTAAVVASLAGKANSIATIFTLDIYKKVLRTDATEKNLVTTGKISIIVAMILGVLIAP 408

Query: 420 QLRQLDQ--AFQYIQEYTGFVSPGVFAIFIFGFFWKKTTSNAALTAAVLTIPLSAAFKVI 477
            L  +D+   FQYIQEYTGFVSPG+FA+FI GFFWK+TTS AAL A +    LS   K +
Sbjct: 409 HL-GIDKKGGFQYIQEYTGFVSPGIFAMFILGFFWKRTTSTAALFATIGGFGLSILLKFL 467

Query: 478 TPNL----------------------PFIDRMGVVFLVLSVLIIAISLYEGKGKDSKKAI 515
            PNL                      PF+DRMG VF+   + +  IS+   K +   K +
Sbjct: 468 -PNLTDLSWLSGMGFSVKNAAGVYEIPFLDRMGFVFVFCIIGMYIISMLSNKAEAEAKGL 526

Query: 516 EVDAELFSTSTKFKVGAVLICGILVALYSVFW 547
            +DA++F TST F VGA++I G+LVALYSV+W
Sbjct: 527 AIDAKMFKTSTSFAVGALIIIGLLVALYSVYW 558


Lambda     K      H
   0.326    0.140    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 950
Number of extensions: 44
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 547
Length of database: 558
Length adjustment: 36
Effective length of query: 511
Effective length of database: 522
Effective search space:   266742
Effective search space used:   266742
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources