GapMind for catabolism of small carbon sources

 

Protein GFF3742 in Phaeobacter inhibens BS107

Annotation: FitnessBrowser__Phaeo:GFF3742

Length: 497 amino acids

Source: Phaeo in FitnessBrowser

Candidate for 13 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arabinose catabolism xacF hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 53% 100% 518.5
D-galacturonate catabolism dopDH hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 53% 100% 518.5
D-glucuronate catabolism dopDH hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 53% 100% 518.5
D-xylose catabolism dopDH hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 53% 100% 518.5
L-valine catabolism mmsA lo methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 31% 96% 251.5 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
L-isoleucine catabolism iolA lo malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 33% 96% 233 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
myo-inositol catabolism mmsA lo malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 33% 96% 233 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
propionate catabolism iolA lo malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 33% 96% 233 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
L-threonine catabolism iolA lo malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 33% 96% 233 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
L-valine catabolism iolA lo malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 33% 96% 233 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
L-lysine catabolism amaB lo Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 (characterized) 32% 91% 228.8 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
L-arginine catabolism astD lo Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) (characterized) 31% 95% 206.5 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5
L-citrulline catabolism astD lo Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) (characterized) 31% 95% 206.5 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 53% 518.5

Sequence Analysis Tools

View GFF3742 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSIPMETEFCVRPLTMTDIQKNLIAGEWLAGESEVENRNPSDLSDLVGIFAQASADQLEA
TLDQAQVAQREWAAYGLERKQAVLNAIGNEMMARAEELGTLLSREEGKPLAEGKGEVYRA
GQFFTYYAAECLRQIGENADSVRPDIEVDVRREAVGTVAIISPWNFPTATASWKIAPALC
YGNAVVWKPANITPASAVALAEIIERQDIPKGLFSLVMGSGRSIGQRLVESPKVNAISFT
GSVPVGKGIASAAIQNLTKVQMEMGSKNALAVMDDADLDLAVTLALGGAFGGTGQKCTAS
SRLVVHAGIHDAFVEKLVTGAKAMKVGHALEAGVQMGPVVSEQQLNENLAYVDLGKTEGA
ELACGGQRLEMPHQGFYMSPGVFLNTTNDMRINREEMFAPLTSVIKVGSYDEALSVVNDT
NFGLTSGIVTQSLARATHFRRNARTGVVTVNLPTAGTDYHVPFGGRGDSSYGPREQGKAA
AEFYTTVKTAYISAGPV

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory