GapMind for catabolism of small carbon sources

 

Protein GFF3831 in Phaeobacter inhibens BS107

Annotation: PGA1_262p02350 histidine transport ATP-binding protein HisP

Length: 281 amino acids

Source: Phaeo in FitnessBrowser

Candidate for 26 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism artP hi AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter (characterized) 64% 98% 314.3 NocP aka ATU6028 aka AGR_PTI_69, component of Nopaline porter 62% 303.5
L-histidine catabolism BPHYT_RS24015 med ABC transporter related (characterized, see rationale) 60% 96% 303.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-lysine catabolism hisP med ABC transporter for L-Lysine, ATPase component (characterized) 64% 96% 301.6 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-histidine catabolism hisP med Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 60% 97% 298.5 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-citrulline catabolism AO353_03040 med ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) 60% 98% 294.3 ABC transporter for L-Arginine, putative ATPase component 62% 312.8
L-citrulline catabolism PS417_17605 med ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale) 58% 97% 290.4 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
D-glucosamine (chitosamine) catabolism AO353_21725 med ABC transporter for D-glucosamine, ATPase component (characterized) 51% 99% 253.1 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-histidine catabolism bgtA med BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) 53% 97% 252.7 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-asparagine catabolism glnQ med Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) 54% 96% 248.1 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-glutamate catabolism gltL med Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) 54% 96% 248.1 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-asparagine catabolism bgtA med ATPase (characterized, see rationale) 50% 99% 240.7 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-aspartate catabolism bgtA med ATPase (characterized, see rationale) 50% 99% 240.7 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-asparagine catabolism bztD med BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 48% 94% 235.3 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-aspartate catabolism bztD med BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized) 48% 94% 235.3 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-histidine catabolism aapP med ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized) 46% 98% 229.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-asparagine catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 46% 99% 229.2 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-aspartate catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 46% 99% 229.2 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-glutamate catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 46% 99% 229.2 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-leucine catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 46% 99% 229.2 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-proline catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 46% 99% 229.2 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
D-alanine catabolism Pf6N2E2_5405 med ABC transporter for D-Alanine, ATPase component (characterized) 47% 99% 223.4 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-asparagine catabolism aatP med Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized) 48% 99% 219.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-aspartate catabolism aatP med Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized) 48% 99% 219.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-asparagine catabolism peb1C med PEB1C, component of Uptake system for glutamate and aspartate (characterized) 46% 98% 209.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-aspartate catabolism peb1C med PEB1C, component of Uptake system for glutamate and aspartate (characterized) 46% 98% 209.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
L-histidine catabolism PA5503 med Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) 40% 72% 171 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3

Sequence Analysis Tools

View GFF3831 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MPDPAAADPAPLSTASAPLQARAETPVEPHTQAEAIRVCDLHKSFGSLEVLKGVSLTAKQ
GDVVAIIGGSGSGKSTMLRCINFLETPNSGEIVIAGETVAMRQDGSPADRRQIERIRTRL
AMVFQQFNLWTHRTLLENVIEVPVHVLKVPRSEAIHRAHELLARVGLGDKADAFPAFLSG
GQQQRAAIARALAVDPNVMLFDEPTSALDPELVGEVLTVIRDLAAEGRTMLLVTHEMKFA
REVANHVVYLFEGRIEEQGPPSEVFGNPKSERLKQFLSSVA

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory