Alignments for a candidate for gguA in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for gguA in Phaeobacter inhibens BS107

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate GFF2274 PGA1_c23060 ribose import ATP-binding protein RbsA

Query= TCDB::O05176
         (512 letters)



>FitnessBrowser__Phaeo:GFF2274
          Length = 503

 Score =  361 bits (926), Expect = e-104
 Identities = 204/501 (40%), Positives = 305/501 (60%), Gaps = 14/501 (2%)

Query: 5   ILEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTYEGEI 64
           +L +  I KTFPGV+AL+ V+L +  GE+HAL+GENGAGKSTLMKVL G++     EG+I
Sbjct: 7   VLRLEGIVKTFPGVRALDGVSLTILPGEVHALMGENGAGKSTLMKVLGGIHQPD--EGQI 64

Query: 65  HYEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASN-GVISWQQTFNR 123
                        D++  GI+ IHQEL+L   LS+AENI+LG       G++ W +   +
Sbjct: 65  IVAEQPVVMSGPLDAKAKGIVFIHQELSLADELSVAENIYLGELPRKRFGLVDWAELEAK 124

Query: 124 TRELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDSEALL 183
           T  +L+K+ +  + +T + D+ +  QQ+VEIA+AL+   K +I DEPTASL +++   L 
Sbjct: 125 TNAILEKLKVGFNAKTRVGDLSIANQQMVEIARALTVDAKAVIFDEPTASLTDAEKVVLF 184

Query: 184 NLLMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIRNMVG 243
            ++ + + QG+    I+H++ E+ K+ D+I+VLRDG    T++    E +E+ + + M+G
Sbjct: 185 EVISDLQEQGVGIAYISHRMEEIFKITDRISVLRDGQYQGTVNT--AETNEENVTQMMIG 242

Query: 244 RDLEDRYPPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLMGAGR 303
           R L+         +GE  LEV+  +          +  D+N  VR+GEVVG  GL+GAGR
Sbjct: 243 RKLDLSRNEAHHELGEVALEVRGLSC-------GSLFEDVNFEVRRGEVVGFYGLVGAGR 295

Query: 304 TEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAGLAYVTEDRKHLGLVLNDNILH 363
           TE A ++FG    +  +G + +DG  V +++   AI+ G++ V EDRK  GLVL  N   
Sbjct: 296 TEIAETLFGLR--NPTSGSIFLDGAEVAITSPHDAIERGISLVPEDRKGQGLVLGMNCRD 353

Query: 364 NTTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGGNQQKVVLSKWL 423
           N TL  +  +     + D  E+ +   +R +L IR+ G  Q   NLSGGNQQK+V+ KWL
Sbjct: 354 NMTLPQVDDLKAGPFVADGAEIAIFDQYRDKLDIRTPGWKQLVGNLSGGNQQKIVIGKWL 413

Query: 424 FSNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELLGNCDRIYVMNE 483
              P+VLI+DEPTRGIDVG+K EI+ ++  LAA G  V++ISSEMPE+L   DRI  M  
Sbjct: 414 SMRPNVLIVDEPTRGIDVGSKAEIHNLLRDLAAQGYAVIVISSEMPEVLHVADRIVAMYS 473

Query: 484 GRIVAELPKGEASQESIMRAI 504
           GRI+      E ++E+++ AI
Sbjct: 474 GRIMRTFTSEEVTEENLIAAI 494



 Score = 65.5 bits (158), Expect = 4e-15
 Identities = 56/238 (23%), Positives = 111/238 (46%), Gaps = 27/238 (11%)

Query: 278 QVLHDINVTVRKGEVVGIAGLMGAGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRK 337
           + L  +++T+  GEV  + G  GAG++   M V G  +     G +++  +PV +S    
Sbjct: 21  RALDGVSLTILPGEVHALMGENGAGKSTL-MKVLGGIH-QPDEGQIIVAEQPVVMSGPLD 78

Query: 338 AIDAGLAYVTEDRKHLGLVLNDNILHNTTLANLAGVSKASIID----------DIKEMKV 387
           A   G+ ++ ++   L L    ++  N  L  L    +  ++D           ++++KV
Sbjct: 79  AKAKGIVFIHQE---LSLADELSVAENIYLGELPR-KRFGLVDWAELEAKTNAILEKLKV 134

Query: 388 ASDFRTRLRIRSSGIFQETVNLSGGNQQKVVLSKWLFSNPDVLILDEPTRGIDVGAKYEI 447
             + +TR+            +LS  NQQ V +++ L  +   +I DEPT  +    K  +
Sbjct: 135 GFNAKTRVG-----------DLSIANQQMVEIARALTVDAKAVIFDEPTASLTDAEKVVL 183

Query: 448 YTIINQLAADGKGVLMISSEMPELLGNCDRIYVMNEGRIVAELPKGEASQESIMRAIM 505
           + +I+ L   G G+  IS  M E+    DRI V+ +G+    +   E ++E++ + ++
Sbjct: 184 FEVISDLQEQGVGIAYISHRMEEIFKITDRISVLRDGQYQGTVNTAETNEENVTQMMI 241


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 648
Number of extensions: 38
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 503
Length adjustment: 34
Effective length of query: 478
Effective length of database: 469
Effective search space:   224182
Effective search space used:   224182
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources