GapMind for catabolism of small carbon sources

 

Finding step artQ for L-arginine catabolism in Phaeobacter inhibens BS107

4 candidates for artQ: L-arginine ABC transporter, permease component 2 (ArtQ/HisQ/AotQ)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med PGA1_262p02370 putative histidine transport system permease protein HisQ ABC transporter for L-Arginine and L-Citrulline, permease component 2 (characterized) 44% 94% 179.9 NocQ aka ATU6026 aka AGR_PTI_66, component of Nopaline porter 42% 174.9
med PGA1_c11560 putative arginine transport system permease protein arginine/ornithine transport protein (characterized) 31% 85% 115.2
lo PGA1_c26600 amino acid ABC transporter, permease protein Histidine transport system permease protein HisQ (characterized) 33% 97% 108.6 Basic amino acid uptake transporter, BgtAB 40% 164.5
lo PGA1_65p00120 putative amino-acid ABC transporter, permease protein ABC transporter for L-Arginine and L-Citrulline, permease component 1 (characterized) 32% 96% 102.4 Probable amino-acid permease protein YxeN 48% 206.1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step artQ

Or cluster all characterized artQ proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory