GapMind for catabolism of small carbon sources


Finding step peb1C for L-asparagine catabolism in Phaeobacter inhibens BS107

4 candidates for peb1C: aspartate ABC transporter, ATPase component Peb1C

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med PGA1_c02010 glutamate/glutamine/aspartate/asparagine transport ATP-binding protein BztD PEB1C, component of Uptake system for glutamate and aspartate (characterized) 58% 99% 280.4 BztD, component of Glutamate/glutamine/aspartate/asparagine porter 83% 445.3
med PGA1_262p02350 histidine transport ATP-binding protein HisP PEB1C, component of Uptake system for glutamate and aspartate (characterized) 46% 98% 209.9 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 64% 314.3
med PGA1_c11580 octopine permease ATP-binding protein P PEB1C, component of Uptake system for glutamate and aspartate (characterized) 43% 98% 205.7 Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR 57% 297.4
med PGA1_65p00130 putative amino-acid ABC transporter, ATP-binding protein PEB1C, component of Uptake system for glutamate and aspartate (characterized) 45% 98% 204.9 Probable amino-acid import ATP-binding protein YxeO; EC 7.4.2.- 55% 274.6

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.

Also see fitness data for the candidates

Definition of step peb1C

Or cluster all characterized peb1C proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory