GapMind for catabolism of small carbon sources

 

Alignments for a candidate for SMc04258 in Phaeobacter inhibens BS107

Align ABC transporter for D-Cellobiose and D-Salicin, permease component 2 (characterized)
to candidate GFF3853 PGA1_78p00170 putative sn-glycerol-3-phosphate transport system permease protein UgpA

Query= reanno::Smeli:SMc04258
         (302 letters)



>FitnessBrowser__Phaeo:GFF3853
          Length = 310

 Score =  454 bits (1167), Expect = e-132
 Identities = 216/293 (73%), Positives = 258/293 (88%), Gaps = 1/293 (0%)

Query: 10  RPNQWLRNLNAKIASIPMILTAMVIFVGGTAWTVVYSFTNSKLLPRLAFVGFDQYERLWA 69
           RP + LRNLNAKIAS+PMILTA+V+F+GGTAWTV +SFT S+LLP+  FVGFDQYERLW+
Sbjct: 19  RPPRVLRNLNAKIASVPMILTALVVFMGGTAWTVAHSFTKSRLLPKWKFVGFDQYERLWS 78

Query: 70  APRWLVSIQNLAVFGCLSLVFSLVIGFVLAALMDQKIRFENTFRTIMLYPFALSFIVTGL 129
           + RWL+S++NL ++G  SLV ++ IGF LAAL+D+KIRFE  FRTI LYPFALSF+VTGL
Sbjct: 79  SNRWLISVENLLIYGLCSLVLTMAIGFTLAALLDRKIRFEGAFRTIFLYPFALSFVVTGL 138

Query: 130 VWQWLLNPQYGIQSIVRSLGWTSFSFDPLYNSNIVIYGILIAALWQGTGLVMCLMLAGLR 189
            WQW+LNP +GIQ++VRS GW SF+FDPL N   VI+G+LIA LWQG+G VM +MLAGLR
Sbjct: 139 AWQWILNPDFGIQNVVRSWGWESFAFDPLNNPETVIFGVLIAGLWQGSGFVMVIMLAGLR 198

Query: 190 GIDEDIWKAARVDGIPMWKTYVLIIIPMMRGVFITTLVIIASGIVKVYDLVVAQTSGGPG 249
           GIDEDIWKAARVDGI + KTYV +IIPMMR VF+T LVIIASGI+K+YDLVVAQT+GGPG
Sbjct: 199 GIDEDIWKAARVDGIGVTKTYVRVIIPMMRPVFVTALVIIASGIIKLYDLVVAQTNGGPG 258

Query: 250 IASEVPAKYVYDYMFQAQNLGQGFAASTMMLVTVAIIIVPWAYLEFGGGRKRG 302
           I+SEVPAKYV +YMF+AQNLGQGFAASTMML++V II+VPWAYLEF GG+KRG
Sbjct: 259 ISSEVPAKYVINYMFEAQNLGQGFAASTMMLLSVIIILVPWAYLEF-GGKKRG 310


Lambda     K      H
   0.329    0.142    0.449 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 446
Number of extensions: 20
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 302
Length of database: 310
Length adjustment: 27
Effective length of query: 275
Effective length of database: 283
Effective search space:    77825
Effective search space used:    77825
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory