Aligments for a candidate for davT in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Aligments for a candidate for davT in Phaeobacter inhibens BS107

Align 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 (characterized)
to candidate GFF3422 PGA1_c34750 putative aminotransferase class 3

Query= SwissProt::P50457
         (421 letters)



>lcl|FitnessBrowser__Phaeo:GFF3422 PGA1_c34750 putative
            aminotransferase class 3
          Length = 1009

 Score =  144 bits (364), Expect = 9e-39
 Identities = 121/399 (30%), Positives = 179/399 (44%), Gaps = 19/399 (4%)

Query: 34   LKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKIN 93
            L D  G  Y+D  A   V + GH HP + A    QL++       + P ++    AEKI 
Sbjct: 615  LFDEWGRPYLD--AYNNVPHVGHAHPRIQAIAADQLKRMNSNTRYLHPAQN--AFAEKI- 669

Query: 94   ALAPVSGQAKTAFFT-TGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVA 152
             L+ +    +  FF  +G EA E A+++ARAHTG  G++    G+HG T   + ++    
Sbjct: 670  -LSKMPDHLEVCFFVNSGTEANELALRLARAHTGAKGMVTPDHGYHGNTTGAIDISAYKF 728

Query: 153  PYKIGFGPFPGSVYHVPYPSDLHGISTQDSLDAIER--------LFKSDIEAKQVAAIIF 204
              K G GP    V  V    D  G   +D     ++        + K       VA  I 
Sbjct: 729  NAKGGVGPSDW-VELVEVADDYRGTYGRDDPQRAQKYADLVDPAIAKLQASGHGVAGFIA 787

Query: 205  EPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQSGFARTGKL-FAMDHYADKPDLM 263
            E     GG  + PK  + A+       G + IADEVQ+G  R G+  F  +H    PD++
Sbjct: 788  ETFPSVGGQIIPPKGYLPAVYEKIRAAGGICIADEVQTGLGRLGEYYFGFEHQGASPDIV 847

Query: 264  TMAKSLAGGMPLSGVVGNANIMDAPAPGG-LGGTYAGNPLAVAAAHAVLNIIDKESLCER 322
             + K +  G PL  +V    I D+ A G     T+ G+ L+      VLNI+D+E L E 
Sbjct: 848  VLGKPIGNGHPLGVLVTTRAIADSFAQGPEFFSTFGGSTLSCRIGTEVLNIVDEEGLQEN 907

Query: 323  ANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQG 382
            A Q G  L N L D +    AI  VRG+G  I VE      G  ++ I   ++ R     
Sbjct: 908  ARQRGADLLNGLRDLQSRHQAIGDVRGMGLFIGVELIRTD-GSEASEICAYVKNRMRDHR 966

Query: 383  LLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALSD 421
            +L+ + G   N+++   PLTI     +  +K L   LS+
Sbjct: 967  ILIGSEGPKDNILKIRPPLTIDAEGIEMILKTLDSILSE 1005


Lambda     K      H
   0.319    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 991
Number of extensions: 49
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 421
Length of database: 1009
Length adjustment: 38
Effective length of query: 383
Effective length of database: 971
Effective search space:   371893
Effective search space used:   371893
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources