Aligments for a candidate for etoh-dh-nad in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Aligments for a candidate for etoh-dh-nad in Phaeobacter inhibens BS107

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate GFF2991 PGA1_c30400 alcohol dehydrogenase AlkJ

Query= BRENDA::Q76HN6
         (526 letters)



>lcl|FitnessBrowser__Phaeo:GFF2991 PGA1_c30400 alcohol dehydrogenase
           AlkJ
          Length = 529

 Score =  376 bits (965), Expect = e-108
 Identities = 218/525 (41%), Positives = 298/525 (56%), Gaps = 4/525 (0%)

Query: 1   MEFDYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHVNW 60
           M +DY+IVGAGSAGCVLANRLSA     V LLEAG +D    +H P+G    +     +W
Sbjct: 1   MSWDYIIVGAGSAGCVLANRLSA-AGQRVLLLEAGGKDNYHWVHIPMGYLYCINNPRTDW 59

Query: 61  AFKTTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDVLP 120
            ++T  + GL GR    PRGKVLGG SSINGM+Y+RG   D++ W+  G  GWG+DDVLP
Sbjct: 60  MYRTEAEAGLNGRALIYPRGKVLGGCSSINGMLYLRGQAADYDGWRQRGLTGWGWDDVLP 119

Query: 121 YFRKSEMHHGGSSEYHGGDGELYVSPANRH-AASEAFVESALRAGHSYNPDFNGATQEGA 179
           YF+KSE +  GSS+ HG  GE  V     H    + ++++A   G     DFN    EG 
Sbjct: 120 YFKKSEDYVDGSSDMHGVGGEWRVENQRLHWDVLDDWMQAAAEWGLPKVTDFNTGNNEGV 179

Query: 180 GYYDVTIRDGRRWSTATAFLKPVRHRSNLTVLTHTHVESIVLLGKQATGVQALIKGSRVH 239
           GY+ V  R G R +TA AFL+      NL V T  H   I++   +A GV+    G+   
Sbjct: 180 GYFRVNQRSGWRMNTAKAFLRTATGE-NLKVETGAHTRRILIENGRAVGVEYSQGGAVKT 238

Query: 240 LRARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDHADVVLCYK 299
            R   EV+LSAGA  SP +L LSG+G  A L   GIA + ++P VGQNLQDH  +   ++
Sbjct: 239 ARTGGEVLLSAGAINSPQILQLSGLGPEALLRDHGIAVQRDMPEVGQNLQDHLQLRCAWR 298

Query: 300 SNDTSLLGFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDPGLERPDIQLHSVI 359
                 L    +  +   K   +YA  R+GP++   ++ GAF ++ P L  PD++ H   
Sbjct: 299 LKGAKTLNTLANSLIGKAKIAAEYAMRRSGPMSMAPSQLGAFSRSRPDLATPDLEYHVQP 358

Query: 360 GTVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHDDDVATLLK 419
            T++   + LH   G +  VC LRP+S G V + S DP +APRI PN+L+ + D    + 
Sbjct: 359 LTLEAFGQPLHDFPGLTASVCNLRPESRGEVAITSADPMQAPRIAPNYLSTEGDRQVAVA 418

Query: 420 GYRITRDIIAQTPMASFGLRDMYSAGLHND-EQLIELLRKRTDTIYHPIGTCKMGQDEMA 478
             R  R I+ Q  M  +   +M   G  ++   L++       TI+HP  T +MG +E A
Sbjct: 419 AIRQARAIMGQEAMQRYAPVEMKPGGGSDEAADLMQAAGAIGTTIFHPTCTLRMGAEEAA 478

Query: 479 VVDSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWI 523
            VD  LR+ G+ GLRVVDAS+MP +  GNTNA  IMIAE+AA+ I
Sbjct: 479 PVDGALRLRGVGGLRVVDASVMPVIPSGNTNAPTIMIAEKAADMI 523


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 770
Number of extensions: 34
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 529
Length adjustment: 35
Effective length of query: 491
Effective length of database: 494
Effective search space:   242554
Effective search space used:   242554
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources