Aligments for a candidate for frcA in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Aligments for a candidate for frcA in Phaeobacter inhibens BS107

Align ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale)
to candidate GFF385 PGA1_c03960 ribose import ATP-binding protein RbsA

Query= uniprot:A0A0C4Y5F6
         (540 letters)



>FitnessBrowser__Phaeo:GFF385
          Length = 509

 Score =  278 bits (712), Expect = 3e-79
 Identities = 188/519 (36%), Positives = 280/519 (53%), Gaps = 22/519 (4%)

Query: 11  APLLALRNICKTFPGVRALRKVELTAYAGEVHALMGENGAGKSTLMKILSGAYTADPGGE 70
           AP + L+ I K F  V+A + + +    G +H ++GENGAGKSTLM IL G Y AD G E
Sbjct: 3   APAIELKGISKAFGPVQANKDISIRVAPGTIHGIIGENGAGKSTLMSILYGFYKADKG-E 61

Query: 71  CHIDGQRVQIDGPQSARDLGVAVIYQELSLAPNLSVAENIYLGRALQRRGLVARGDMVRA 130
             I G+R +I   Q+A   G+ +++Q   L  N +V ENI LG      G + +  + +A
Sbjct: 62  VWIHGKRTEIPDSQAAISAGIGMVFQHFKLVENFTVLENIILGA---EDGGLLKPSLSKA 118

Query: 131 CAPTLARLGADFS----PAANVASLSIAQRQLVEIARAVHFEARILVMDEPTTPLSTHET 186
              +L  L A++     P A +  + +  +Q VEI +A++ +A IL++DEPT  L+  E 
Sbjct: 119 -RKSLKDLAAEYELNVDPDARIDEIGVGMQQRVEILKALYRQADILILDEPTGVLTPAEA 177

Query: 187 DRLFALIRQLRGEGMAILYISHRMAEIDELADRVTVLRDGCFVGTLDRAHLSQAALVKMM 246
           D+LF ++ +LR EG  I+ I+H++ EI E  D V+V+R G    T+  A  S   L ++M
Sbjct: 178 DQLFRILDRLRAEGKTIILITHKLREIMEYTDTVSVMRRGEMTATVKTAETSPEHLAELM 237

Query: 247 VGRDLSGFYTK---THGQAVEREVMLSVRDVADGRRVKGCSFDLRAGEVLGLAGLVGAGR 303
           VGR +     K   T G+ +     LSV D A   RVK     +RAGE+LG+AG+ G G+
Sbjct: 238 VGRKVLLRVDKVPATPGKPILEIENLSVVDEAGVARVKNIDLTVRAGEILGIAGVAGNGQ 297

Query: 304 TELARLVFGADARTRGEVRIANPAGSGGLVTLPAGGP--RQAIDAGIAYLTEDRKLQGLF 361
           +EL   V G     +G +R+     +G  + L   G   R    A +A++ EDR+ +GL 
Sbjct: 298 SELME-VLGGMREGQGSIRL-----NGAPLPLSGAGSDARARRAAHVAHVPEDRQREGLI 351

Query: 362 LDQSVHENINLIVAARDALGLGRLNRTAARRRTTEA-IDTLGIRVAHAQVNVGALSGGNQ 420
           +D    EN+            G L   AA R  TEA +    +R     +     SGGNQ
Sbjct: 352 MDFHAWENVAFGYHHAPEYQRGLLMNNAALRADTEAKMAKFDVRPPDPWLAAKNFSGGNQ 411

Query: 421 QKVMLSRLLEIQPRVLILDEPTRGVDIGAKSEIYRLINALAQSGVAILMISSELPEVVGL 480
           QK++++R +E  P +L++ +PTRGVDIGA   I++ I  L   G AIL++S EL E++ L
Sbjct: 412 QKIVVAREIERNPELLLIGQPTRGVDIGAIEFIHKQIVELRDQGKAILLVSVELEEILSL 471

Query: 481 CDRVLVMREGTLAGEVRPAGSAAETQERIIALATGAAAA 519
            DRV VM +G + GE RPA    E +  ++       AA
Sbjct: 472 ADRVAVMFDGMIMGE-RPADQTDEKELGLLMAGVAGEAA 509


Lambda     K      H
   0.320    0.136    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 625
Number of extensions: 34
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 509
Length adjustment: 35
Effective length of query: 505
Effective length of database: 474
Effective search space:   239370
Effective search space used:   239370
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources