GapMind for catabolism of small carbon sources

 

Aligments for a candidate for PfGW456L13_1894 in Phaeobacter inhibens BS107

Align ABC transporter for D-Galactose and D-Glucose, periplasmic substrate-binding component (characterized)
to candidate GFF1918 PGA1_c19500 putative sugar-binding periplasmic protein

Query= reanno::pseudo13_GW456_L13:PfGW456L13_1894
         (432 letters)



>lcl|FitnessBrowser__Phaeo:GFF1918 PGA1_c19500 putative
           sugar-binding periplasmic protein
          Length = 414

 Score =  220 bits (560), Expect = 7e-62
 Identities = 148/424 (34%), Positives = 205/424 (48%), Gaps = 23/424 (5%)

Query: 13  LASLSALPLSVLAAESKGSVEVVHWWTSGGEKAAVDVLKAQVEKDGFTWKDGAVAGGGGS 72
           LA ++ L ++  A       EV+HWW S GE AA+ V       +G  W D  + GGG  
Sbjct: 8   LAGVATLAMTSAATADGIRAEVIHWWVSAGEAAAIKVFADAYTANGGEWIDNGIGGGG-- 65

Query: 73  TAMTVLKSRAVAGNPPGVAQIK-GPDIQEWGSTGLLSTDALKDVSKAENWDGLLSKKVSD 131
            A T   +R + G+ P V Q     + +E    GLL +  L   ++A NW  L    + +
Sbjct: 66  -AKTTFVNRLMGGDAPQVGQFNTSREFEEIVDAGLLHS--LDAEAEAGNWSALFPGIIDN 122

Query: 132 TVKYEGDYVAVPVNIHRVNWLWINPEVFKKAGIEKAPTTLEEFYAAGDKLKAAGFIALAH 191
            VK +G Y AVPVNIH  NWLW N +V   AG++  PT  + F+ A + LK AG I LA 
Sbjct: 123 VVKRDGSYYAVPVNIHGSNWLWHNNQVMADAGLD-VPTDWDSFFEAAETLKEAGIIPLAV 181

Query: 192 GGQPWQDSTVFEDVVLSVMGADGYKKALVDLDQKTLSGPEMTKSF---AELKKITGYMDP 248
           GG+ WQ+   F  V+LSV G D Y +   + D   L+  EM + F   + L+ +    DP
Sbjct: 182 GGEAWQERLTFNSVLLSVGGQDLYLRLFEEKDTTALTSDEMKEVFDVYSRLRTLVRETDP 241

Query: 249 NRAGRDWNIAAADVISGKAGMQMMGDWAKSEWTAAKKIAGKDYQCVAFPGTEKAFTYNID 308
              GR WN A   VI+G+A MQ+MGDWAK E+ +A    G +Y C   P       Y I 
Sbjct: 242 GSPGRSWNDATNMVITGQAAMQIMGDWAKGEFLSAGMTPGVEYGCT--PAVIAGSPYMIS 299

Query: 309 SMAVFKLKADRKGDIAAQQDLAKVALGTDFQKVFSMNKGSIPVRNDMLNEMDKLGFDECA 368
                  K   + D  AQ  +A   L  + Q  F+  KGSIPVR D    +D    D C 
Sbjct: 300 GDVFVFPKTGNEEDREAQSLMATTMLDAEVQVAFNNIKGSIPVRPD----VDTSQLDVCG 355

Query: 369 QKS-AKDFIADDKTGGLQPSMAHNMATSLAVQGAIFDVVTNFMNDKDADPAKASAQLASA 427
           Q++ A     D   G  Q  ++ ++A      GA+ DV T F N +     +A   L+ A
Sbjct: 356 QQAIALTSNPDTHVGVTQMYISSDLA------GALQDVYTQFWNSETMTTEEAITLLSQA 409

Query: 428 VKAA 431
            + A
Sbjct: 410 YEIA 413


Lambda     K      H
   0.314    0.129    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 467
Number of extensions: 20
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 432
Length of database: 414
Length adjustment: 32
Effective length of query: 400
Effective length of database: 382
Effective search space:   152800
Effective search space used:   152800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory