Alignments for a candidate for glpV in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpV in Phaeobacter inhibens BS107

Align GlpV, component of Glycerol uptake porter, GlpSTPQV (characterized)
to candidate GFF511 PGA1_c05230 ABC transporter, extracellular solute-binding protein

Query= TCDB::G3LHZ3
         (569 letters)



>FitnessBrowser__Phaeo:GFF511
          Length = 577

 Score =  711 bits (1835), Expect = 0.0
 Identities = 352/575 (61%), Positives = 428/575 (74%), Gaps = 19/575 (3%)

Query: 4   TAGLLLAMTA--SAYAGMDEAKTFLDKEVGDLSTLPRADQEKEMQWFVDAAKPFAGMDIK 61
           +A  ++A+ A  +AYA    AK ++D+E    S L + +Q  EMQWF+ A++PF+GM+I 
Sbjct: 7   SAAAVIAVVAGQAAYADEAAAKKWIDEEFQP-SVLTKDEQLSEMQWFIKASEPFSGMEIN 65

Query: 62  VVSETITTHEYESKVLAPAFTAITGIKITHDLIGEGDVVEKLQTQMQSGENIYDAYINDS 121
           V+SE I TH YES+VL  AF  ITGIK+ H ++GEG+VV+ +QTQMQ+  N+YDAY+NDS
Sbjct: 66  VLSEGIPTHSYESEVLTKAFEEITGIKVNHQILGEGEVVQAVQTQMQTKRNLYDAYVNDS 125

Query: 122 DLIGTHWRYQQARSLTDWMANEGKDVTNPGLDIDDFIGKSFTTAPDGKLYQLPDQQFANL 181
           DLIGTH R Q A +LTD M  +  DVTNP LD+ DF+G  FTT PDG LYQLPDQQFANL
Sbjct: 126 DLIGTHSRLQLAYNLTDMMEGDFFDVTNPELDLGDFMGTQFTTGPDGDLYQLPDQQFANL 185

Query: 182 YWFRYDWFNDEKNKADFKAKYGYDLGVPVNWSAYEDIAEFFTG--REIDGKKVFGHMDYG 239
           YWFR DWF+ E  +A FK KYGYDLGVPVNWSAYEDIAEFF+   +EIDG  +FGHMDYG
Sbjct: 186 YWFRKDWFDREDLQAAFKEKYGYDLGVPVNWSAYEDIAEFFSNDVKEIDGTTIFGHMDYG 245

Query: 240 KKDPSLGWRFTDAWLSMAGNGDKGIPNGKPVDEWGIKVDENS-RPVGSCVARGGDTNGPA 298
           K+ P LGWR TDAWLSMAG G KG PNG P+DEWGI+++ +S  P G+ V RGG  NGPA
Sbjct: 246 KRAPDLGWRMTDAWLSMAGAGSKGEPNGVPIDEWGIRMEADSCNPAGASVTRGGAANGPA 305

Query: 299 AVYSIQKYLDWMKAYAPAAAQGMTFSESGPVPSQGEVAQQMFTYTAFTADFVK---EGLP 355
           AVY+I+K+ +W++ +AP  A    F +S P  +QG VAQQ+F YTAFTAD VK   EG  
Sbjct: 306 AVYAIRKWDEWLRKFAPPGAASYDFYQSLPALAQGNVAQQIFWYTAFTADMVKPQSEGNN 365

Query: 356 VVNADGTPKWRFAPSPHGVYWKDGMKLGYQDAGSWTLMKSTPDDRAKAAWLYAQFVTSKT 415
            V+ +G P WR APSPHG YW++G K+GYQD GSWT +KSTP DRAKAAWLYAQFV SKT
Sbjct: 366 TVDGEGNPLWRMAPSPHGPYWEEGQKVGYQDVGSWTFLKSTPMDRAKAAWLYAQFVVSKT 425

Query: 416 VDVKKSHMGLTFIRQSTLDHKSFTDRAPKLGGLIEFYRSPARLQWSPTGTNVPDYPKLAQ 475
           VDVKKSH+GLTFIR S+++H+SFT+RAPKLGGL+EFYRSP R+ WSPTG NVPDYPKLAQ
Sbjct: 426 VDVKKSHVGLTFIRDSSVNHESFTERAPKLGGLVEFYRSPDRVAWSPTGVNVPDYPKLAQ 485

Query: 476 LWWQAIGDASSGAKTAQEAMDSLCSEQEKVLQRLER----AKVQGDIGPKLAEEHDLAYW 531
           +WWQ IGD +SGA T QEAMD L  E +  + R++R    A V G  GP+L EE D  +W
Sbjct: 486 IWWQQIGDVNSGAFTPQEAMDRLAEEMDITMARMQRADEQASVYGGCGPRLNEEKDAEWW 545

Query: 532 NADAVKNGNLAPQLKIENEKDKPVTVNYDELVKSW 566
            A    NG   P  K+ENEK +  TVNYDELV  W
Sbjct: 546 YA----NGGAKP--KLENEKPQGQTVNYDELVARW 574


Lambda     K      H
   0.315    0.132    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1285
Number of extensions: 57
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 569
Length of database: 577
Length adjustment: 36
Effective length of query: 533
Effective length of database: 541
Effective search space:   288353
Effective search space used:   288353
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources