GapMind for catabolism of small carbon sources

 

Alignments for a candidate for BPHYT_RS24010 in Phaeobacter inhibens BS107

Align Polar amino acid ABC transporter, inner membrane subunit (characterized, see rationale)
to candidate GFF3909 PGA1_65p00120 putative amino-acid ABC transporter, permease protein

Query= uniprot:B2TBJ8
         (250 letters)



>FitnessBrowser__Phaeo:GFF3909
          Length = 224

 Score =  102 bits (255), Expect = 5e-27
 Identities = 67/227 (29%), Positives = 115/227 (50%), Gaps = 10/227 (4%)

Query: 4   DFDFLFDTIKQLLAAVPTTLGLFFCSLILGGLLSLVIVTMRVSPHWLPNRFARAYILVFR 63
           D  ++   +  LL+ VP TL +   +++L  +L+ ++   RV    + ++    +I  FR
Sbjct: 5   DIAYMLGLVPVLLSYVPLTLFMAVVAMVLALVLASLLAVERVLRIPVLDQLVVLFISFFR 64

Query: 64  GSPLLIQMFLVYYGMGQFGVIRESFLWPVLREPYMCAVLSLALCTAGYTAEIIRGGLMAV 123
           G+PLL+Q+FL YYG+ Q   +               A++ L L  A Y AE IR  ++ V
Sbjct: 65  GTPLLVQLFLFYYGLPQVLSVLTQI------NGVSAAIMGLTLHFAAYMAESIRAAILGV 118

Query: 124 PVGQIEAGYSIGLSGFALLRRVIGPIALRQCLPAYSTEAVLLVKSTALASLVTVWEVTGV 183
              Q EA  SIG++   ++RR++ P A R   P      + ++K T+LA  + V E+ G 
Sbjct: 119 DRSQWEAAQSIGMTRGQMMRRIVLPQAARIAAPTLVNYFIDMIKGTSLAFTLGVTEMMGA 178

Query: 184 AQQIIQQTYRTTEVFICAALIYLFLNFVIVRLLGMLETRLSRHLRAA 230
            Q+    ++   E F+  A+IY    +++V  L +++ RL  HL  A
Sbjct: 179 TQKEAAGSFLYFEAFLVVAVIY----WILVEALSLVQRRLETHLNKA 221


Lambda     K      H
   0.331    0.143    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 121
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 250
Length of database: 224
Length adjustment: 23
Effective length of query: 227
Effective length of database: 201
Effective search space:    45627
Effective search space used:    45627
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory