GapMind for catabolism of small carbon sources

 

Alignments for a candidate for dgoA in Phaeobacter inhibens BS107

Align 2-dehydro-3-deoxy-phosphogluconate aldolase (EC 4.1.2.14); 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16); (4S)-4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.42) (characterized)
to candidate GFF2758 PGA1_c28010 KHG/KDPG aldolase

Query= BRENDA::P0A955
         (213 letters)



>FitnessBrowser__Phaeo:GFF2758
          Length = 215

 Score =  207 bits (528), Expect = 9e-59
 Identities = 105/207 (50%), Positives = 138/207 (66%), Gaps = 1/207 (0%)

Query: 6   TSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVP 65
           T A  I    P+VPV+VV  + HA P+A+AL+AGG+  LEVTLRT  A+D I  +AK V 
Sbjct: 10  TRARDICQLAPIVPVLVVHDVAHARPLAEALIAGGLPALEVTLRTPAALDVIAEMAK-VK 68

Query: 66  EAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGM 125
             +VGAGT++ P+ +     AGAQF +SPG T+ LL AA    +P++PG +T SE M  +
Sbjct: 69  GGVVGAGTLITPEDVTRAVAAGAQFGVSPGATDTLLDAAEAAGLPMLPGAATASEAMRLL 128

Query: 126 DYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLCIGGSW 185
           D G    KFFPAEA+GG  AL+AI  P  Q+ FCPTGG+SP N   YL+L +V+C GGSW
Sbjct: 129 DRGYDMLKFFPAEASGGAPALKAIGAPLPQISFCPTGGVSPDNADSYLSLSNVVCAGGSW 188

Query: 186 LVPADALEAGDYDRITKLAREAVEGAK 212
           + PA  +EAGD+D I  LAR A + A+
Sbjct: 189 VAPAKLVEAGDWDGIRDLARAASQLAR 215


Lambda     K      H
   0.317    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 182
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 213
Length of database: 215
Length adjustment: 22
Effective length of query: 191
Effective length of database: 193
Effective search space:    36863
Effective search space used:    36863
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory