Align Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 (characterized)
to candidate GFF2122 PGA1_c21540 propionyl-CoA carboxylase alpha chain
Query= SwissProt::Q99MR8 (717 letters) >FitnessBrowser__Phaeo:GFF2122 Length = 681 Score = 501 bits (1290), Expect = e-146 Identities = 283/693 (40%), Positives = 404/693 (58%), Gaps = 43/693 (6%) Query: 47 KVLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAM 106 K+LIANRGEIACRVI+TA+KMG+++VA+YS+AD+ S+HV MADEA IGP P+ QSY+ + Sbjct: 4 KILIANRGEIACRVIKTARKMGIKTVAIYSDADKQSLHVKMADEAVHIGPPPANQSYIVI 63 Query: 107 EKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSSAIRDMGIKSTSKSIM 166 +K+++ +++ AQA+HPGYGFLSEN +FAE EG+ F+GPP AI MG K TSK I Sbjct: 64 DKVMEAIRATGAQAVHPGYGFLSENAKFAEALAAEGVAFVGPPVGAIESMGDKITSKKIA 123 Query: 167 AAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKGMRIVRSEREFQEQLES 226 AGV V GY G + + +IGYPVMIKA GGGGKGMRI S+ E +E +S Sbjct: 124 QEAGVSTVPGYMGLIADADEAVKISNEIGYPVMIKASAGGGGKGMRIAWSDAEAREGFQS 183 Query: 227 ARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAP 286 ++ EA SF DD + IEKFV PRH+E+QV D HGN +YL ER+CS+QRR+QK++EEAP Sbjct: 184 SKNEAANSFGDDRIFIEKFVTQPRHIEIQVLCDAHGNGIYLGERECSIQRRNQKVVEEAP 243 Query: 287 APGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDSRHNFYFMEMNTRLQVEHPVTEMI 346 +P ++ + RR +GE AV AKAV Y AGTVEFI+D NFYF+EMNTRLQVEHPVTE+I Sbjct: 244 SPFLDEDTRRAMGEQAVALAKAVGYASAGTVEFIVDGDKNFYFLEMNTRLQVEHPVTELI 303 Query: 347 TGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHL----- 401 TG DLVE +RIA GE + ++Q ++ L G A E R+YAEDP F+P G L Sbjct: 304 TGVDLVEQMIRIAGGEPLSITQNDVKLNGWAIENRLYAEDPYRGFLPSIGRLTRYRPPAE 363 Query: 402 --------------STPSADMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKL 447 P+ +++ R +TGV +G E+S++YDPMIAKL W R +A++ + Sbjct: 364 VAAGPLHDSGKWQGDAPAGELAVRNDTGVFEGGEISMYYDPMIAKLCTWGPTRDAAIAAM 423 Query: 448 RYCLHQYNIVGLRSNVDFLLRLSGHPEFEAGNVHTDFIPQHHKD-----LLPSHSTIAKE 502 R L + + G+ N+ FL + HP F AG + T FI + + + LP + K Sbjct: 424 RDALDGFEVEGIGHNLPFLSAVMDHPIFIAGTMTTAFIEEQYPEGFNGVELPEQD-LRKI 482 Query: 503 SVCQAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKSDIVIAV 562 + AA+ + + + + ++ + R++ + +TL+ + + V Sbjct: 483 AAASAAMHRVAEIRRTRVSGRMDNHE----------RKVGSDWV--VTLQDVSFN--VTV 528 Query: 563 TYNRDGSYDMQIDNKSFRVLGDLSSEDGCTYLKSSINGVARKSKFILLDNTVHLFSMEGS 622 +RDGS D + RV D + D L + + K I + + Sbjct: 529 DADRDGSTVRFDDGSALRVASDWTPGDQLARLTVGDDSLVVKVGKISGGFRIRSRGADLK 588 Query: 623 IEVGIPVPKYLSPVSAEGAQGGT----IAPMTGTIEKVFVKAGDRVKAGDSLMVMIAMKM 678 + V P L+ + E T + PM G + K+ V+ GD V+ G +L + AMKM Sbjct: 589 VHVRTPRQAELAKLMPEKIAPDTSKLLLCPMPGLVVKLEVEVGDEVQEGQALCTIEAMKM 648 Query: 679 EHTIKAPKDGRIKKVFFSEGAQANRHAPLVEFE 711 E+ ++A K G + + + G ++EFE Sbjct: 649 ENILRAEKKGVVSAINAAAGDSLAVDEVIMEFE 681 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1104 Number of extensions: 41 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 717 Length of database: 681 Length adjustment: 39 Effective length of query: 678 Effective length of database: 642 Effective search space: 435276 Effective search space used: 435276 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory