GapMind for catabolism of small carbon sources

 

Alignments for a candidate for atoD in Phaeobacter inhibens BS107

Align acetyl-CoA:acetoacetate CoA transferase, B subunit (EC 2.8.3.8) (characterized)
to candidate GFF606 PGA1_c06200 probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B

Query= reanno::psRCH2:GFF1044
         (209 letters)



>FitnessBrowser__Phaeo:GFF606
          Length = 209

 Score =  304 bits (779), Expect = 7e-88
 Identities = 153/207 (73%), Positives = 178/207 (85%)

Query: 1   MAWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWLQSENGLLGIGPFPTEE 60
           MAW R QMA RAAQELQDG+YVNLGIG+PTLV+N+IPEG++V LQSENG+LG+GPFP E 
Sbjct: 1   MAWDRNQMAARAAQELQDGWYVNLGIGIPTLVSNHIPEGVNVTLQSENGMLGMGPFPVEG 60

Query: 61  EIDPDLINAGKQTVTALPGSSFFDNAQSFAMIRGGHINLAILGAMQVSEKGDLANWMIPG 120
           E D DLINAGKQT+T LP +++FD+AQSFAMIRGG I +AILGAM+V+E GDLANWMIPG
Sbjct: 61  EEDADLINAGKQTITELPQTAYFDSAQSFAMIRGGKIAMAILGAMEVAENGDLANWMIPG 120

Query: 121 KMVKGMGGAMDLVAGVKRVVVLMEHTAKGGAHKILPACDLPLTGLGVVDRIITDLGVLDV 180
           K+VKGMGGAMDLVAGV RVVV+M+HT K G  K+L  C LPLTG GVVDRIIT+LGVLDV
Sbjct: 121 KLVKGMGGAMDLVAGVGRVVVVMDHTNKHGDSKVLTDCTLPLTGKGVVDRIITNLGVLDV 180

Query: 181 TEQGLKLVELAEGVSFDELQEATGSPI 207
            + GLK+VE AEGVS DEL+ AT + I
Sbjct: 181 VDGGLKIVETAEGVSEDELRAATQATI 207


Lambda     K      H
   0.318    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 254
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 209
Length of database: 209
Length adjustment: 21
Effective length of query: 188
Effective length of database: 188
Effective search space:    35344
Effective search space used:    35344
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory