Align Branched-chain amino acid ABC transporter permease LivH; SubName: Full=Branched-chain amino acid transporter permease subunit LivH; SubName: Full=L-leucine ABC transporter membrane protein /L-isoleucine ABC transporter membrane protein /L-valine ABC transporter membrane protein (characterized, see rationale)
to candidate GFF247 PGA1_c02590 putative high-affinity branched-chain amino acid transport system permease protein LivH
Query= uniprot:A0A0D9B2B6 (307 letters) >FitnessBrowser__Phaeo:GFF247 Length = 291 Score = 155 bits (391), Expect = 1e-42 Identities = 97/301 (32%), Positives = 158/301 (52%), Gaps = 16/301 (5%) Query: 9 QQLVNGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGLAMMGLDSV 68 Q LV+GL G Y LIA+G+ ++Y +NFA G+ M+G++V GL + Sbjct: 5 QLLVSGLANGCVYGLIALGFVLIYKATEAVNFAQGDFMMLGAFVTL----GLTNAEYLHL 60 Query: 69 PLLMTAAFIASIVVTSSYGYSIERIAYRPLRGSNR--LIPLISAIGMSIFLQNTVLLSQD 126 P + A SI + + GY ++ + R L G N+ ++ L A+G I + + Sbjct: 61 PFWLAAPL--SIAIMAGLGYLLDLVILRHLFGQNQTAVVILTIALGFVIRFFAGAIWGHE 118 Query: 127 SKDKSIPNLIPGNFAIGPGGAHEVLISYMQIVVFVVTLVAMLGLTLFISRSRLGRACRAC 186 P + A+G V++ + + +VT++ L F R++LG A +A Sbjct: 119 ------PQTLESPLALGDVQLAGVVLGLADLAIIIVTVLLTWSLYQFFQRTKLGLAMQAA 172 Query: 187 AEDIKMANLLGINTNNIIALTFVIGAALAAIAAVLLSMQYGVINPNAGFLVGLKAFTAAV 246 +++ A +GI + L + + A AA+A +L + + G I+PNAG L+G+KAF AAV Sbjct: 173 SQNQMAAYFMGIPVKRVQGLIWGLSGATAAVAGILFASK-GAIDPNAG-LLGIKAFAAAV 230 Query: 247 LGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILGRPEVEK 306 +GG GS+PGA+ GGL++GV E F A Y + + LL+ VL+FRP G+ + +K Sbjct: 231 IGGFGSLPGALAGGLIVGVIEPFAARYLAAGYSQIAPYVLLLAVLVFRPHGLFSQVRTKK 290 Query: 307 V 307 V Sbjct: 291 V 291 Lambda K H 0.327 0.144 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 236 Number of extensions: 15 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 307 Length of database: 291 Length adjustment: 27 Effective length of query: 280 Effective length of database: 264 Effective search space: 73920 Effective search space used: 73920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory