Alignments for a candidate for paaK in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaK in Phaeobacter inhibens BS107

Align medium-chain acyl-CoA ligase (EC 6.2.1.2); phenylacetate-CoA ligase (EC 6.2.1.30) (characterized)
to candidate GFF2497 PGA1_c25300 long-chain-fatty-acid--CoA ligase FadD

Query= BRENDA::O74725
         (578 letters)



>FitnessBrowser__Phaeo:GFF2497
          Length = 577

 Score =  142 bits (359), Expect = 3e-38
 Identities = 147/539 (27%), Positives = 243/539 (45%), Gaps = 55/539 (10%)

Query: 49  KSYSSKEVANRVDSLARSLSKEFGWAPNEGSEWDKTLAVFALNTIDSLPLFWAVHRLGGV 108
           K+Y+  E+    D++A  L ++ G  P +        A+   N+I    + +   + G  
Sbjct: 71  KTYA--EIGRASDAIACYLREDLGLFPGD------VAAIQLPNSIHYPVILFGAMKAGLK 122

Query: 109 LTPANASYSAAELTHQLLDSKAKALVTCVPLLSISLEAAAKAGLPKNRIYLLDVPEQLLG 168
           +T  N  Y+  E+ HQL DS AK L     L +  L+ A +    +  +         L 
Sbjct: 123 VTNLNPLYTPREIVHQLQDSGAKVLFG-FNLFADRLQTALRDTAVEQVVIAA------LW 175

Query: 169 GVKPPAGYKSVSE-LTQAGKSLPPVD-----------------ELRWSAGE-GARRTAFV 209
              P A   ++   +T   K +P                    E+ +   +      AF+
Sbjct: 176 EFFPQAASDALRHTITDVAKLVPEYSFDHVPFEDAFAQGMAHGEIHYDLSQIDPGSAAFL 235

Query: 210 CYSSGTSGLPKGVMISHRNVIANTLQIKAFEQNYRDGGGTKPASTEVA-LGLLPQSHIYA 268
            Y+ GT+G+ KG  ++H NV      + A       G   K    +++ L ++P  HI+A
Sbjct: 236 QYTGGTTGVSKGAELTHDNVTHVLEMLNACVH----GAMQKTDHGQMSILTVIPYYHIFA 291

Query: 269 LVV-IGHAGAYRGDQTIVLPKFELKSYLNAIQQYKISALFLVPPIIIHMLGTQDVCSKYD 327
           L+V + H  A  G   ++     + +   A   Y I  L  V  +   ++  QD   +  
Sbjct: 292 LIVNLMHFTAASGRNVLIPNPNPIANLKPAFDNYDIDWLTGVDTLFNGLMA-QDWFQQTP 350

Query: 328 LSSVTSLFTGAAPLGMETAADFLKLYPNILIRQGYGLTET-CTVVSSTHPHDIWLGSSGA 386
              +     G   L  +TA  + +    I+  +GYG+TET C +  S        G++G 
Sbjct: 351 -PQIGLAIGGGTSLRPDTARRWSEQIGPIV--EGYGMTETTCMIALSPLDGSDKPGTAGQ 407

Query: 387 LLPGVEARIVTPENKEITTYDSPGELVVRSPSVVLGYLNNEKATAETFVDGWMRTGDEAV 446
            +PG+E +I   +   +   ++ GEL VR P++   YLN   A+AETF DGWM TGD  +
Sbjct: 408 PVPGLEIKITRSDGAPVGIGEA-GELHVRGPNIATAYLNRPDASAETFADGWMATGD--I 464

Query: 447 IRRSPKGIEHVFIVDRIKELIKVKGLQVAPAELEAHILAHPDVSDCAVIAIPDDRAGEVP 506
           +    +G  H+ IVDR K++I V G  V P ELEA I + P V++ AV+       GE P
Sbjct: 465 VTMDAEG--HLAIVDRKKDMILVSGFNVYPNELEAVIQSMPGVAEVAVVGEAHPTRGESP 522

Query: 507 KAIVVKSASAGSDESVSQALVKYVEDHKARHKWLKGGIRFVDAIPKSPSGKILRRLIRD 565
            A V ++  A ++E V    +KY   +   +K +   + F++ +PKS  GKILR+ +R+
Sbjct: 523 AAFVKRADPAVTEEMV----IKYCRANLTAYK-VPTQVHFLEELPKSSVGKILRKELRN 576


Lambda     K      H
   0.316    0.134    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 721
Number of extensions: 40
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 578
Length of database: 577
Length adjustment: 36
Effective length of query: 542
Effective length of database: 541
Effective search space:   293222
Effective search space used:   293222
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources