Alignments for a candidate for ectP in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for ectP in Phaeobacter inhibens BS107

Align Glycine betaine/proline/choline transporter VP1723 (characterized)
to candidate GFF1371 PGA1_c13880 putative transporter, BCCT family

Query= SwissProt::Q87NZ5
         (553 letters)



>FitnessBrowser__Phaeo:GFF1371
          Length = 545

 Score =  770 bits (1988), Expect = 0.0
 Identities = 371/539 (68%), Positives = 439/539 (81%), Gaps = 1/539 (0%)

Query: 6   NGGIKRPDGKVNAIDTDYQIGQDNVALKVGPFGLDIHNRVFAISGMAIVLFVVATLTFRQ 65
           N GI  PDG+   IDT+Y+IGQDN+   VGP G DIHN VF +SG++I+LFV   L   +
Sbjct: 7   NQGIPAPDGEAAVIDTEYEIGQDNLEGSVGPIGFDIHNPVFMVSGISIMLFVFYALVLPE 66

Query: 66  QVEPFFAGLRAWLVSNLDWFFLASGNVFVIVCLVLIVTPLGRVRIGGTEATPDYSYAGWL 125
           Q   FF  LR  + S+ DWFFL++GN FV+ C  LIV+P G+VR+GG +A PDY+Y GW 
Sbjct: 67  QAAAFFGWLRPAVTSSFDWFFLSAGNFFVLFCFFLIVSPWGKVRLGGADAAPDYTYTGWF 126

Query: 126 AMLFAAGMGIGLVFFGVSEPMSHFSSALGGVNI-ENGVRTDWAPLGGAVGDTDAASALGM 184
           AMLFAAGMGIGL+F+GVSEPMSH+S++ GGV++ ENG RTDWAPLGGA GD+  +  LGM
Sbjct: 127 AMLFAAGMGIGLMFYGVSEPMSHYSTSFGGVSMGENGARTDWAPLGGAAGDSAESVRLGM 186

Query: 185 AATIYHWALHPWSIYALLALGLAIFSFNKGLPLTMRSIFYPLFGERVWGWVGHIIDILAV 244
           AATIYHW LHPW+IYA++AL LA+FSFNKGLPLT+RS FYP+FGERVWGW GHIIDILAV
Sbjct: 187 AATIYHWGLHPWAIYAIVALSLALFSFNKGLPLTIRSAFYPIFGERVWGWTGHIIDILAV 246

Query: 245 VATVFGLATSLGYGASQAATGLNFLFGVPMTDTTQVVLIVVITALALISVVAGLDSGVKR 304
            AT+FGLATSLG+GA+QA  GLN LFGVP+  TT+VVLI  ITA+ALISV+ GLD GVK 
Sbjct: 247 FATLFGLATSLGFGATQANAGLNELFGVPVGATTEVVLISAITAVALISVLRGLDGGVKI 306

Query: 305 LSEINMILAAMLLFFVIIVGPTMAILTGFFDNIASYITNIPALSMPFEREDVNYSQGWTA 364
           LSEINM LA +LL FV++VGPT+ I+TGFFD++ +Y+  +PALSMPF RED NYSQGWTA
Sbjct: 307 LSEINMGLAFLLLIFVLLVGPTLLIITGFFDSLLAYVQYLPALSMPFGREDANYSQGWTA 366

Query: 365 FYWAWWISWSPFVGMFIARVSRGRSVREFIICVILIPSTVCVLWMTAFGGTAISQYVNDG 424
           FYWAWWISWSPFVGMFIARVSRGR+VREFIICV+LIPS VCVLWM+ FGGTAI Q V DG
Sbjct: 367 FYWAWWISWSPFVGMFIARVSRGRTVREFIICVLLIPSLVCVLWMSVFGGTAIHQVVADG 426

Query: 425 YEAVFNAELPLKLFAMLDVMPFAEITSVVGIILVVVFFITSSDSGSLVIDTIAAGGKVDA 484
           Y    +  L LKLF MLD +P A ITS VGI+LV+VFF+TSSDSGSLVIDTI AGGKVDA
Sbjct: 427 YTGAQDTALELKLFKMLDQLPLAAITSFVGILLVIVFFVTSSDSGSLVIDTITAGGKVDA 486

Query: 485 PTPQRVFWCTFEGLVAIALMLGGGLAAAQAMAVTTGLPFTIVLLVATVSLIKGLMDEPR 543
           P PQRVFWC FEG VAI L+LGGGL A QAM ++TGLPFT+VLL+   ++++GL  E R
Sbjct: 487 PMPQRVFWCVFEGAVAIVLLLGGGLVALQAMVISTGLPFTVVLLLMCWAIVRGLQTETR 545


Lambda     K      H
   0.327    0.141    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1019
Number of extensions: 50
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 553
Length of database: 545
Length adjustment: 36
Effective length of query: 517
Effective length of database: 509
Effective search space:   263153
Effective search space used:   263153
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources