Alignments for a candidate for prpE in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Phaeobacter inhibens BS107

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate GFF1279 PGA1_c12950 acetyl-coenzyme A synthetase Acs

Query= SwissProt::P27550
         (652 letters)



>FitnessBrowser__Phaeo:GFF1279
          Length = 695

 Score =  830 bits (2145), Expect = 0.0
 Identities = 396/633 (62%), Positives = 483/633 (76%), Gaps = 5/633 (0%)

Query: 16  RCLINPQQYEAMYQQSINVPDTFWGEQGKILDWIKPYQKVKNTSFAPGNVSIKWYEDGTL 75
           R  +N  QY+ MY  S+  P+ FW +Q + +DWIKP+ +VK+  F  GNVSI WY DGTL
Sbjct: 61  RAHVNAAQYDEMYAASMQDPEGFWRQQAERIDWIKPFTQVKDVDFTLGNVSINWYADGTL 120

Query: 76  NLAANCLDRHLQENGDRTAIIWEGDDASQS-KHISYKELHRDVCRFANTLLELGIKKGDV 134
           N+AANC+DRHL   GD+TAIIWE D   ++ +HISYK+LH  VCR AN L  +G++KGD 
Sbjct: 121 NVAANCIDRHLDTRGDQTAIIWEPDSPDEAAQHISYKQLHTRVCRMANVLETMGVRKGDR 180

Query: 135 VAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGR 194
           V IY+PM+PEAA AMLACARIGA+HS++F GFSP+A+A R+   +++++IT+DE  R GR
Sbjct: 181 VVIYLPMIPEAAYAMLACARIGAIHSIVFAGFSPDALAARVNGCDAKVLITADEAPRGGR 240

Query: 195 SIPLKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMN 254
             PLK N D AL +   T     +V+KRTGG+  W +GRD  ++++  +A D  +  EMN
Sbjct: 241 KTPLKSNADAALLHTKDTV--KCLVVKRTGGQTTWIDGRDYDYNEMALEADDYSKPAEMN 298

Query: 255 AEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHS 314
           AEDPLFILYTSGSTG+PKGV+HTTGGYL YAA+T +  FDYH GDIYWCTADVGWVTGHS
Sbjct: 299 AEDPLFILYTSGSTGQPKGVVHTTGGYLTYAAMTHEITFDYHDGDIYWCTADVGWVTGHS 358

Query: 315 YLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEG 374
           Y++YGPLA GATTLMFEGVP +P  +R  QV DKH+V   YTAPTA+RALM +G++ +E 
Sbjct: 359 YIVYGPLANGATTLMFEGVPTYPDASRFWQVCDKHKVTQFYTAPTALRALMGQGNEWVEK 418

Query: 375 TDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKA 434
            D SSLR LG+VGEPINPEAW WY   +G  KCP+VDTWWQTETGG ++TPLPGA   K 
Sbjct: 419 CDLSSLRTLGTVGEPINPEAWNWYNDIVGKGKCPIVDTWWQTETGGHLMTPLPGAHATKP 478

Query: 435 GSATRPFFGVQPALVD-NEGNPLEG-ATEGSLVITDSWPGQARTLFGDHERFEQTYFSTF 492
           G+A +PFFGV P ++D   G  + G   EG L I DSWPGQ RT++GDHERFE+TYFS +
Sbjct: 479 GAAMKPFFGVAPVVLDPQSGEEITGNGVEGVLCIKDSWPGQMRTVWGDHERFEKTYFSDY 538

Query: 493 KNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPH 552
           K  YF+GDG RRDEDG YWITGRVDDV+NVSGHR+GTAE+ESALVAH  +AEAAVVG PH
Sbjct: 539 KGYYFTGDGCRRDEDGDYWITGRVDDVINVSGHRMGTAEVESALVAHAAVAEAAVVGYPH 598

Query: 553 NIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMR 612
            IKGQ IY YVTL +  EPS EL  E+R WVR EIGP+A+PDV+ W   LPKTRSGKIMR
Sbjct: 599 EIKGQGIYCYVTLMNDREPSDELVKELRTWVRTEIGPIASPDVIQWAPGLPKTRSGKIMR 658

Query: 613 RILRKIAAGDTSNLGDTSTLADPGVVEKLLEEK 645
           RILRKIA  D  +LGDTSTLADP VV+ L+  +
Sbjct: 659 RILRKIAENDFGSLGDTSTLADPSVVDDLIANR 691


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1525
Number of extensions: 64
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 695
Length adjustment: 39
Effective length of query: 613
Effective length of database: 656
Effective search space:   402128
Effective search space used:   402128
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources