Align Alr3027 protein, component of The 2-oxo monocarboxylate transporter (Pernil et al., 2010). Transports pyruvate which is inhibited by various 2-ketoacids (characterized)
to candidate GFF1982 PGA1_c20160 TRAP transporter, subunit DctM
Query= TCDB::Q8YSQ7 (445 letters) >FitnessBrowser__Phaeo:GFF1982 Length = 785 Score = 156 bits (394), Expect = 3e-42 Identities = 129/381 (33%), Positives = 184/381 (48%), Gaps = 53/381 (13%) Query: 90 LLETMGILLGRLRG------GLALAVVLVGALLAATTGVVAATV-----VAMGLISLPIM 138 L+ MG++L RG L + +G LL A VVA + + LI+LP + Sbjct: 428 LMVLMGLVLAVGRGVSPSSDPKPLILGAIGVLLIALVDVVAIAPTTSPGITVLLIALPTL 487 Query: 139 LRYGYNKELATGVIAASGTLGQIIPPSVVLV-VLGDQLGISVGDLFIGSVIPGLMMASAF 197 L Y +A G A + + + PP +++V VLG LG G P A Sbjct: 488 LAL-YGCRIAAGRSARNDLIRVVFPPLILIVAVLGSILG--------GITNPTPAAALGA 538 Query: 198 ALHVLIVAFIRPDVAPALPAQVREIGGKALGKRVIQVMIPPLILILLVLGSIFFGFATPT 257 +++ A+ + + GK+ GK +I I IL+ + F Sbjct: 539 GGAIMLAAYRK-----------LQDQGKS-GKIIIWATFAVAICILV---GVNFDLRING 583 Query: 258 EAGAVGCAGAIALAAANGQFTLES---------------LRQVCDTTLRITSMVVFILIG 302 +G V IA A G + L V T ++TSMV ILIG Sbjct: 584 RSG-VSAETVIAFGVAYGAYLFALFGLLYGCWVLFKGGVLTPVVRETAKVTSMVFTILIG 642 Query: 303 STAFSLVFRGLNGDQFMFDVLANLPGGKIGFLFVSMTTVFLLGFFIDFFEIAFIVIPLFV 362 S +LV G+ ++ L + FL V M +F+LGF +DF EI +IVIP+ Sbjct: 643 SQLLNLVVISFGGEHYIQQFLKSFDSELKVFLIV-MVVLFILGFVLDFLEIIYIVIPIVG 701 Query: 363 PVAQKLGIDLVWYGVILGANLQTSFLTPPFGFALFYLRGVAPPEVTTSDIYRGVIPFILL 422 PV D W +++ NLQTSFLTPPFGFALFYLRGVAP EVTT+ IYRGV+PF+L+ Sbjct: 702 PVIYGGSFDPKWVTIMVAVNLQTSFLTPPFGFALFYLRGVAPKEVTTAHIYRGVLPFVLI 761 Query: 423 QLLVLLLIIIFPGIVSFLPSL 443 Q++ L ++ +FP IV+ +P+L Sbjct: 762 QVVGLGILWMFPSIVTIVPAL 782 Score = 153 bits (387), Expect = 2e-41 Identities = 88/189 (46%), Positives = 118/189 (62%), Gaps = 28/189 (14%) Query: 55 MPQRIFGIMANYTLLAIPYFIFMGAMLEKSGIAERLLETMGILLGRLRGGLALAVVLVGA 114 M +R+ + TLLA+ F+ MG LE+S IA LL TM + G L GGLA+++V+VGA Sbjct: 113 MNERVLAGASIETLLAVLMFVLMGITLERSKIANDLLTTMARVFGPLPGGLAVSIVVVGA 172 Query: 115 LLAATTGVVAATVVAMGLISLPIMLRYGYNKELATGVIAASGTLGQIIPPSVVLVVLGDQ 174 LAA+TG+V ATVV MGL++LP MLR Y+ ELATGVIAASGTLGQIIPPS+V+V+LG Sbjct: 173 FLAASTGIVGATVVTMGLLALPTMLRNNYSPELATGVIAASGTLGQIIPPSIVIVLLGTL 232 Query: 175 LG----------------------------ISVGDLFIGSVIPGLMMASAFALHVLIVAF 206 G +SVG LF +++PG+M+A +AL+ A Sbjct: 233 AGDLYSTAQEARAQEFGCSDALTYLGEPAVVSVGTLFQAALLPGIMLALLYALYAFGYAL 292 Query: 207 IRPDVAPAL 215 + P+ APA+ Sbjct: 293 LNPEKAPAV 301 Lambda K H 0.331 0.149 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 820 Number of extensions: 41 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 3 Length of query: 445 Length of database: 785 Length adjustment: 37 Effective length of query: 408 Effective length of database: 748 Effective search space: 305184 Effective search space used: 305184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory