Align Alpha-glucosidase; EC 3.2.1.- (characterized, see rationale)
to candidate GFF775 PGA1_c07890 putative alpha-glucosidase AglA
Query= uniprot:A8LLL3 (552 letters) >FitnessBrowser__Phaeo:GFF775 Length = 552 Score = 845 bits (2184), Expect = 0.0 Identities = 391/551 (70%), Positives = 446/551 (80%), Gaps = 1/551 (0%) Query: 1 MNAEAQMREVKSLAADPDWWRGAVIYQIYPRSFQDSNGDGIGDLLGIVERMPYIASLGVD 60 MNA+AQ+ + LAADPDWWRGAVIYQIYPRS+QDSNGDGIGDL GI +R+P+IASLGVD Sbjct: 1 MNAQAQLDPAQVLAADPDWWRGAVIYQIYPRSYQDSNGDGIGDLRGITQRLPHIASLGVD 60 Query: 61 AIWISPFFTSPMKDFGYDISDYFDVDPMFGSLADFDALIETAHMYGLRVMIDLVLSHTSD 120 AIWISPFFTSPMKDFGYD+SDY DVDPMFGSL++FD L+ AH GLRVMIDLVLSHTSD Sbjct: 61 AIWISPFFTSPMKDFGYDVSDYCDVDPMFGSLSNFDQLVAAAHRLGLRVMIDLVLSHTSD 120 Query: 121 QHPWFEESRSSRDNPKADWYVWADAKPDGTPPNNWLSIFGGSGWHWDARRCQYYLHNFLT 180 QH WF ESR SRDN +ADWYVWAD +PDGTPPNNWLSIFGGS W WD RR QYYLHNFL Sbjct: 121 QHAWFGESRQSRDNARADWYVWADPQPDGTPPNNWLSIFGGSAWQWDPRREQYYLHNFLV 180 Query: 181 SQPDLNFHCADVQDALLGVGRFWLDRGVDGFRLDTINFYVHDAELRDNPPLPPEERNSNI 240 SQPDLNFH VQDALL V RFWL+RGVDGFRLDTINFY HDAELR NP LPPE+RN+ I Sbjct: 181 SQPDLNFHSPAVQDALLDVTRFWLERGVDGFRLDTINFYYHDAELRSNPALPPEQRNATI 240 Query: 241 APEVNPYNHQRHLYSKNQPENLEFLAKFRAMMEEYPAIAAVGEVGDAQYGLEILGQYTRG 300 AP VNPYNHQ HLYSKNQPENL FL +FRA+++EYPA AAVGEVGDAQ GLEI+G YT Sbjct: 241 APSVNPYNHQEHLYSKNQPENLAFLGRFRALLDEYPAKAAVGEVGDAQRGLEIMGSYTAA 300 Query: 301 ETGVHMCYAFEFLAQEKLTAKRVAEVLNKVDEVASDGWACWAFSNHDVMRHVSRWDLTPG 360 TGVHMCYAFE LA++ LTA R+AEV +VD VA++GWACWAFSNHDV+RH SRW L P Sbjct: 301 NTGVHMCYAFELLAKDVLTASRLAEVFAEVDRVAANGWACWAFSNHDVIRHSSRWGLNPA 360 Query: 361 AQRGMLTLLMCLRGSVCLYQGEELGLPEAEVAFDDLQDPYGIEFWPEYKGRDGCRTPMVW 420 AQR T++MCLRG+ C+YQGEELGLPEA++AF+DLQDPYGIEFWPE+KGRDGCRTPMVW Sbjct: 361 AQRLFTTMMMCLRGTTCIYQGEELGLPEADIAFEDLQDPYGIEFWPEFKGRDGCRTPMVW 420 Query: 421 QSDNMSGGFSIHRPWLPVSTEHLGLAVAVQEEAPDALLHHYRRALAFRRAHPALVKGDIS 480 + N SGGFS +PWLPVS EHL L+VA QE PDA+LHHYRRA+A R+AHPAL G Sbjct: 421 EPSNGSGGFSAGKPWLPVSPEHLNLSVASQEADPDAMLHHYRRAIALRKAHPALAVGTHD 480 Query: 481 DVTVVGDVISFLRKDPEETVFVAINMSDAPGAVDLPPGNWMQIGAELNSGGTSPDG-RVH 539 + G+V F R+D +E +F A N+ D P + LP G W + ++ G RV Sbjct: 481 QLRAEGNVAFFTRQDRDEVIFCAFNLGDIPAEITLPEGTWRKPDTDIALADLPQSGARVV 540 Query: 540 LGPWQPCIALK 550 L PWQ C+A++ Sbjct: 541 LAPWQACLAVR 551 Lambda K H 0.321 0.138 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1155 Number of extensions: 36 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 552 Length adjustment: 36 Effective length of query: 516 Effective length of database: 516 Effective search space: 266256 Effective search space used: 266256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory