Alignments for a candidate for frcA in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for frcA in Phaeobacter inhibens BS107

Align ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale)
to candidate GFF2274 PGA1_c23060 ribose import ATP-binding protein RbsA

Query= uniprot:A0A0C4Y5F6
         (540 letters)



>FitnessBrowser__Phaeo:GFF2274
          Length = 503

 Score =  357 bits (915), Expect = e-103
 Identities = 218/508 (42%), Positives = 308/508 (60%), Gaps = 17/508 (3%)

Query: 10  KAPLLALRNICKTFPGVRALRKVELTAYAGEVHALMGENGAGKSTLMKILSGAYTADPGG 69
           ++P+L L  I KTFPGVRAL  V LT   GEVHALMGENGAGKSTLMK+L G +  D G 
Sbjct: 4   QSPVLRLEGIVKTFPGVRALDGVSLTILPGEVHALMGENGAGKSTLMKVLGGIHQPDEG- 62

Query: 70  ECHIDGQRVQIDGPQSARDLGVAVIYQELSLAPNLSVAENIYLGRALQRR-GLVARGDMV 128
           +  +  Q V + GP  A+  G+  I+QELSLA  LSVAENIYLG   ++R GLV   ++ 
Sbjct: 63  QIIVAEQPVVMSGPLDAKAKGIVFIHQELSLADELSVAENIYLGELPRKRFGLVDWAELE 122

Query: 129 RACAPTLARLGADFSPAANVASLSIAQRQLVEIARAVHFEARILVMDEPTTPLSTHETDR 188
                 L +L   F+    V  LSIA +Q+VEIARA+  +A+ ++ DEPT  L+  E   
Sbjct: 123 AKTNAILEKLKVGFNAKTRVGDLSIANQQMVEIARALTVDAKAVIFDEPTASLTDAEKVV 182

Query: 189 LFALIRQLRGEGMAILYISHRMAEIDELADRVTVLRDGCFVGTLDRAHLSQAALVKMMVG 248
           LF +I  L+ +G+ I YISHRM EI ++ DR++VLRDG + GT++ A  ++  + +MM+G
Sbjct: 183 LFEVISDLQEQGVGIAYISHRMEEIFKITDRISVLRDGQYQGTVNTAETNEENVTQMMIG 242

Query: 249 RDLSGFYTKTHGQAVEREVMLSVRDVADGRRVKGCSFDLRAGEVLGLAGLVGAGRTELAR 308
           R L     + H +    EV L VR ++ G   +  +F++R GEV+G  GLVGAGRTE+A 
Sbjct: 243 RKLDLSRNEAHHEL--GEVALEVRGLSCGSLFEDVNFEVRRGEVVGFYGLVGAGRTEIAE 300

Query: 309 LVFGADARTRGEVRIANPAGSGGLVTLPAGGPRQAIDAGIAYLTEDRKLQGLFLDQSVHE 368
            +FG    T G + +      G  V + +  P  AI+ GI+ + EDRK QGL L  +  +
Sbjct: 301 TLFGLRNPTSGSIFL-----DGAEVAITS--PHDAIERGISLVPEDRKGQGLVLGMNCRD 353

Query: 369 NINLIVAARDALGLGRLNRTAARRRTTEAI-DTLGIRVAHAQVNVGALSGGNQQKVMLSR 427
           N+ L     D L  G      A     +   D L IR    +  VG LSGGNQQK+++ +
Sbjct: 354 NMTL--PQVDDLKAGPFVADGAEIAIFDQYRDKLDIRTPGWKQLVGNLSGGNQQKIVIGK 411

Query: 428 LLEIQPRVLILDEPTRGVDIGAKSEIYRLINALAQSGVAILMISSELPEVVGLCDRVLVM 487
            L ++P VLI+DEPTRG+D+G+K+EI+ L+  LA  G A+++ISSE+PEV+ + DR++ M
Sbjct: 412 WLSMRPNVLIVDEPTRGIDVGSKAEIHNLLRDLAAQGYAVIVISSEMPEVLHVADRIVAM 471

Query: 488 REGTLAGEVRPAGSAAETQERIIALATG 515
             G +   +R   S   T+E +IA  +G
Sbjct: 472 YSGRI---MRTFTSEEVTEENLIAAISG 496



 Score = 64.3 bits (155), Expect = 1e-14
 Identities = 53/219 (24%), Positives = 98/219 (44%), Gaps = 9/219 (4%)

Query: 39  GEVHALMGENGAGKSTLMKILSGAYTADPGGECHIDGQRVQIDGPQSARDLGVAVIYQEL 98
           GEV    G  GAG++ + + L G       G   +DG  V I  P  A + G++++ ++ 
Sbjct: 282 GEVVGFYGLVGAGRTEIAETLFGLRNPT-SGSIFLDGAEVAITSPHDAIERGISLVPEDR 340

Query: 99  S---LAPNLSVAENIYLGRA--LQRRGLVARGDMVRACAPTLARLGADFSPAAN--VASL 151
               L   ++  +N+ L +   L+    VA G  +        +L    +P     V +L
Sbjct: 341 KGQGLVLGMNCRDNMTLPQVDDLKAGPFVADGAEIAIFDQYRDKLDIR-TPGWKQLVGNL 399

Query: 152 SIAQRQLVEIARAVHFEARILVMDEPTTPLSTHETDRLFALIRQLRGEGMAILYISHRMA 211
           S   +Q + I + +     +L++DEPT  +       +  L+R L  +G A++ IS  M 
Sbjct: 400 SGGNQQKIVIGKWLSMRPNVLIVDEPTRGIDVGSKAEIHNLLRDLAAQGYAVIVISSEMP 459

Query: 212 EIDELADRVTVLRDGCFVGTLDRAHLSQAALVKMMVGRD 250
           E+  +ADR+  +  G  + T     +++  L+  + G D
Sbjct: 460 EVLHVADRIVAMYSGRIMRTFTSEEVTEENLIAAISGLD 498


Lambda     K      H
   0.320    0.136    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 589
Number of extensions: 28
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 540
Length of database: 503
Length adjustment: 35
Effective length of query: 505
Effective length of database: 468
Effective search space:   236340
Effective search space used:   236340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources