Aligments for a candidate for maiA in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Aligments for a candidate for maiA in Phaeobacter inhibens BS107

Align Maleylacetoacetate isomerase (EC 5.2.1.2) (characterized)
to candidate GFF38 PGA1_c00380 putative maleylacetoacetate isomerase

Query= reanno::MR1:200836
         (216 letters)



>lcl|FitnessBrowser__Phaeo:GFF38 PGA1_c00380 putative
           maleylacetoacetate isomerase
          Length = 228

 Score =  162 bits (410), Expect = 5e-45
 Identities = 96/219 (43%), Positives = 132/219 (60%), Gaps = 16/219 (7%)

Query: 1   MILYGYWRSSAAYRVRIALNLKGVSAEQLSVHLVRDGGEQHKADYIALNPQELVPTLVVD 60
           +ILY YWRSSA+YRVRIALNL G+S   ++V LV+  GEQ   D++A NPQ LVP L   
Sbjct: 4   VILYDYWRSSASYRVRIALNLAGISYRAVTVDLVK--GEQVSPDHLARNPQGLVPVL--- 58

Query: 61  DEQDGDALTQSLAIIEYLDELYPKTPLLPASALERAHVRAMALTIACEIHPLNNLRVLQY 120
            E DG  LTQSLAI++YLD+      LLP +  ERA  +A+A  IA ++HP+ NL+V ++
Sbjct: 59  -EIDGLRLTQSLAILDYLDQTR-HLDLLPRTPAERALAQALAHAIAVDLHPVCNLKVARH 116

Query: 121 LTQKLTVNEEAKSA--------WYHHWVATGFTALETQLVRHS-GRYCFGDKVTIADLCL 171
            +   T +  + +A        W  H++  G  A  T L  H    YC GD   +ADLCL
Sbjct: 117 ASDLCTGSAASPAAQPVDMPADWMRHFIRPGLVAFNTLLEEHPVAPYCTGDHPGLADLCL 176

Query: 172 VPQVYNAQRFNVDLTPYPNIMRVWAECNQLPAFADAAPE 210
           +PQ+YNA+R+ V+    P ++ +   C   PAFA A P+
Sbjct: 177 IPQLYNARRWGVNFDDLPRLVTIETTCAGNPAFAMAHPD 215


Lambda     K      H
   0.321    0.134    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 166
Number of extensions: 9
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 216
Length of database: 228
Length adjustment: 22
Effective length of query: 194
Effective length of database: 206
Effective search space:    39964
Effective search space used:    39964
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate GFF38 PGA1_c00380 (putative maleylacetoacetate isomerase)
to HMM TIGR01262 (maiA: maleylacetoacetate isomerase (EC 5.2.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01262.hmm
# target sequence database:        /tmp/gapView.3063.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01262  [M=211]
Accession:   TIGR01262
Description: maiA: maleylacetoacetate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                        -----------
      2e-71  226.0   0.0    2.6e-71  225.6   0.0    1.1  1  lcl|FitnessBrowser__Phaeo:GFF38  PGA1_c00380 putative maleylaceto


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Phaeo:GFF38  PGA1_c00380 putative maleylacetoacetate isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  225.6   0.0   2.6e-71   2.6e-71       1     206 [.       5     216 ..       5     220 .. 0.90

  Alignments for each domain:
  == domain 1  score: 225.6 bits;  conditional E-value: 2.6e-71
                        TIGR01262   1 klYsyfrSsasyRvRiaLaLkgidyesvpvnLlkdGeqkkeefkalNPqelvPtLkidegevltqSlAiieyLeetyp 78 
                                      +lY+y+rSsasyRvRiaL+L gi+y++v+v+L+k Geq ++++ a+NPq+lvP+L+id g +ltqSlAi++yL++t+ 
  lcl|FitnessBrowser__Phaeo:GFF38   5 ILYDYWRSSASYRVRIALNLAGISYRAVTVDLVK-GEQVSPDHLARNPQGLVPVLEID-GLRLTQSLAILDYLDQTRH 80 
                                      59********************************.9**********************.6**************9987 PP

                        TIGR01262  79 epaLlpkdpakrarvralalliacdihPlqNlrvlqlleeklg...vde.....eekkewlkhwiekGlaalEellk. 147
                                         Llp+ pa+ra ++ala++ia d+hP++Nl+v +  ++ +    +++     ++ ++w++h+i+ Gl a+ +ll+ 
  lcl|FitnessBrowser__Phaeo:GFF38  81 -LDLLPRTPAERALAQALAHAIAVDLHPVCNLKVARHASDLCTgsaASPaaqpvDMPADWMRHFIRPGLVAFNTLLEe 157
                                      .89*******************************9987654431112331111134579******************6 PP

                        TIGR01262 148 ekagafcvGdevtladvcLvpqvynAerfevdlaqyPtlkrieealaelpafqeahpen 206
                                      +    +c+Gd+++lad+cL+pq+ynA+r++v++++ P+l +ie+++a  paf+ ahp+ 
  lcl|FitnessBrowser__Phaeo:GFF38 158 HPVAPYCTGDHPGLADLCLIPQLYNARRWGVNFDDLPRLVTIETTCAGNPAFAMAHPDA 216
                                      67889****************************************************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (211 nodes)
Target sequences:                          1  (228 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 7.86
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources