Alignments for a candidate for pccB in Phaeobacter inhibens BS107

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccB in Phaeobacter inhibens BS107

Align Propionyl-CoA carboxylase beta chain; EC 6.4.1.3 (characterized)
to candidate GFF2128 PGA1_c21600 propionyl-CoA carboxylase beta chain

Query= SwissProt::Q168G2
         (510 letters)



>FitnessBrowser__Phaeo:GFF2128
          Length = 510

 Score =  926 bits (2393), Expect = 0.0
 Identities = 450/510 (88%), Positives = 485/510 (95%)

Query: 1   MKDILEQLEDRRAAARLGGGQKRIDAQHGRGKLTARERVDLLLDEGSFEEFDMFVTHRCT 60
           MKDIL +LEDRR AARLGGGQKRIDAQHGRGKLTARER++LLLDEGSFEEFDMFV HRCT
Sbjct: 1   MKDILSELEDRRNAARLGGGQKRIDAQHGRGKLTARERIELLLDEGSFEEFDMFVAHRCT 60

Query: 61  DFNMQDQKPAGDGVVTGWGTINGRVVYVFSQDFTVLGGSVSETHSKKICKIMDMAMQNGA 120
           DF M++Q+PAGDGVVTGWGTINGR+VYVFSQDFTV GGS+SETH++KICKIMDMA+QNGA
Sbjct: 61  DFGMENQRPAGDGVVTGWGTINGRMVYVFSQDFTVFGGSLSETHAQKICKIMDMAVQNGA 120

Query: 121 PVIGINDSGGARIQEGVDSLAGYGEVFQRNIMASGVVPQISMIMGPCAGGAVYSPAMTDF 180
           PVIGINDSGGARIQEGV SLAGY EVFQRNIMASGVVPQIS+IMGPCAGGAVYSPAMTDF
Sbjct: 121 PVIGINDSGGARIQEGVASLAGYAEVFQRNIMASGVVPQISVIMGPCAGGAVYSPAMTDF 180

Query: 181 IFMVKDSSYMFVTGPDVVKTVTNEQVSAEELGGATTHTRKSSVADAAFENDVEALAEVRR 240
           IFMVKD+SYMFVTGPDVVKTVTNE V+AEELGGA+THT+KSSVAD AFENDVEALAEVRR
Sbjct: 181 IFMVKDTSYMFVTGPDVVKTVTNEVVTAEELGGASTHTKKSSVADGAFENDVEALAEVRR 240

Query: 241 LVDFLPLNNREKPPVRPFFDDPDRIEPSLDTLVPDNPNTPYDMKELIHKLADEGDFYEIQ 300
           LVDFLPLNNREKPPVRPFFD+P R+E SLDTL+P NPNTPYDMKELIHK+ADEGDFYEIQ
Sbjct: 241 LVDFLPLNNREKPPVRPFFDEPGRVETSLDTLIPANPNTPYDMKELIHKVADEGDFYEIQ 300

Query: 301 EEFAKNIITGFIRLEGRTVGVVANQPLVLAGCLDIDSSRKAARFVRFCDAFEIPLLTLID 360
           E+FAKNIITGFIRLEG+TVGVVANQP VLAGCLDIDSSRKAARFVRFCD FEIP+LTL+D
Sbjct: 301 EDFAKNIITGFIRLEGQTVGVVANQPTVLAGCLDIDSSRKAARFVRFCDCFEIPILTLVD 360

Query: 361 VPGFLPGTSQEYGGVIKHGAKLLYAYGEATVPMVTVITRKAYGGAYVVMSSKHLRADFNY 420
           VPGFLPGTSQEYGGVIKHGAKLL+AYGEATVP VTVITRKAYGGAY VM+SKHLR DFNY
Sbjct: 361 VPGFLPGTSQEYGGVIKHGAKLLFAYGEATVPKVTVITRKAYGGAYDVMASKHLRGDFNY 420

Query: 421 AWPTAEVAVMGAKGATEIIHRGDLGDPEKIAQHTADYEERFANPFVASERGFVDEVIQPR 480
           AWPTAE+AVMGAKGATEIIHR DL D +KIA+HT DYEERFANPFVA+ERGF+DEVI P+
Sbjct: 421 AWPTAEIAVMGAKGATEIIHRADLADADKIAEHTKDYEERFANPFVAAERGFIDEVIMPQ 480

Query: 481 STRKRVARAFASLRNKSVQMPWKKHDNIPL 510
           STRKRV+RAFASLR K ++ PWKKHDNIPL
Sbjct: 481 STRKRVSRAFASLRGKQLKNPWKKHDNIPL 510


Lambda     K      H
   0.319    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 978
Number of extensions: 30
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 510
Length of database: 510
Length adjustment: 34
Effective length of query: 476
Effective length of database: 476
Effective search space:   226576
Effective search space used:   226576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources