GapMind for catabolism of small carbon sources

 

Protein PP_2047 in Pseudomonas putida KT2440

Annotation: FitnessBrowser__Putida:PP_2047

Length: 412 amino acids

Source: Putida in FitnessBrowser

Candidate for 22 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 48% 57% 366.7 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
phenylacetate catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 48% 57% 366.7 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-phenylalanine catabolism pimF lo 6-carboxyhex-2-enoyl-CoA hydratase (characterized) 48% 57% 366.7 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
4-hydroxybenzoate catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
4-hydroxybenzoate catabolism paaH lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-arginine catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-citrulline catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-lysine catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
phenylacetate catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
phenylacetate catabolism paaH lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-phenylalanine catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-phenylalanine catabolism paaH lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-proline catabolism fadB lo 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized) 42% 57% 313.5 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-isoleucine catabolism ech lo Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 (characterized) 41% 57% 307 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
4-hydroxybenzoate catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-arginine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-citrulline catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-lysine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
phenylacetate catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-phenylalanine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-proline catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7
L-valine catabolism ech lo 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized) 33% 59% 180.3 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) 100% 819.7

Sequence Analysis Tools

View PP_2047 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MNTPFPINQVAVIGAGTMGRGIVISLANAGLSVLWLDCNAAALEAGLGMVSQAWAQQVDK
QRITQAQADACLARVQAVDGYPALAEADLVIEAVYENLALKQEIFCALDAHLKPRAILAS
NTSALDIDAIAAVTQRPSQVLGLHFFSPAHVMKLLEIVRGTHTDQKVLDAAKALGERMGK
VAIVAGNCPGFIGNRMLRSYVGEARKLLLEGALPHQVDAVLQQFGFAMGPFRMYDVVGID
LEWRARQLAGQGMHDPLVQVDNALCELGRLGQKTGQGYYRYAPGSRQAEHDPQVDALVLQ
VSQNLGYRRRGISAEEILERCLLALVNEGAKVLQEGIAASSGDIDQVWLHGYGFPAATGG
PMRWADEQGAPFILARLEYLQGVLGEHWRPAGLLYSLVAGGKRFEPRGEVQA

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory