GapMind for catabolism of small carbon sources

 

Protein PP_2216 in Pseudomonas putida KT2440

Annotation: FitnessBrowser__Putida:PP_2216

Length: 375 amino acids

Source: Putida in FitnessBrowser

Candidate for 18 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism acdH hi short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) (characterized) 100% 100% 736.9
L-valine catabolism acdH hi 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (characterized) 89% 100% 666.8
L-lysine catabolism bcd med butanoyl-CoA dehydrogenase (NAD+, ferredoxin) (subunit 3/3) (EC 1.3.1.109); short-chain acyl-CoA dehydrogenase (EC 1.3.8.1) (characterized) 46% 99% 351.3 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
4-hydroxybenzoate catabolism Ch1CoA med cyclohex-1-ene-1-carbonyl-CoA dehydrogenase (EC 1.3.8.10) (characterized) 41% 98% 275 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-leucine catabolism liuA med acyl-CoA dehydrogenase subunit (EC 1.3.8.4; EC 1.3.8.5) (characterized) 41% 98% 275 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
phenylacetate catabolism Ch1CoA med cyclohex-1-ene-1-carbonyl-CoA dehydrogenase (EC 1.3.8.10) (characterized) 41% 98% 275 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-phenylalanine catabolism Ch1CoA med cyclohex-1-ene-1-carbonyl-CoA dehydrogenase (EC 1.3.8.10) (characterized) 41% 98% 275 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
4-hydroxybenzoate catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-arginine catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-citrulline catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-lysine catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
phenylacetate catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-phenylalanine catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-proline catabolism gcdH lo glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized) 36% 93% 221.9 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-isoleucine catabolism pco lo acyl-CoA oxidase (EC 1.3.3.6) (characterized) 31% 85% 171.8 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
propionate catabolism pco lo acyl-CoA oxidase (EC 1.3.3.6) (characterized) 31% 85% 171.8 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-threonine catabolism pco lo acyl-CoA oxidase (EC 1.3.3.6) (characterized) 31% 85% 171.8 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9
L-valine catabolism pco lo acyl-CoA oxidase (EC 1.3.3.6) (characterized) 31% 85% 171.8 short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) 100% 736.9

Sequence Analysis Tools

View PP_2216 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MLVNDEQQQIADAVRAFAQERLKPFAEQWDKDHRFPKEAIDEMAELGLFGMLVPEQWGGS
DTGYVAYAMALEEIAAGDGACSTIMSVHNSVGCVPILRFGNEQQKEQFLTPLATGAMLGA
FALTEPQAGSDASSLKTRARLEGDHYVLNGSKQFITSGQNAGVVIVFAVTDPEAGKRGIS
AFIVPTDSPGYQVARVEDKLGQHASDTCQIVFDNVQVPVANRLGAEGEGYKIALANLEGG
RIGIASQAVGMARAAFEVARDYANERQSFGKPLIEHQAVAFRLADMATKISVARQMVLHA
AALRDAGRPALVEASMAKLFASEMAEKVCSDALQTLGGYGYLSDFPLERIYRDVRVCQIY
EGTSDIQRMVIARNL

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory