Alignments for a candidate for bamH in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for bamH in Pseudomonas putida KT2440

Align Benzoyl-CoA reductase electron transfer protein, putative (characterized, see rationale)
to candidate PP_2184 PP_2184 putative Formate dehydrogenase, beta subunit

Query= uniprot:Q39TW5
         (635 letters)



>FitnessBrowser__Putida:PP_2184
          Length = 525

 Score =  319 bits (818), Expect = 2e-91
 Identities = 190/523 (36%), Positives = 287/523 (54%), Gaps = 26/523 (4%)

Query: 26  PCISVCAGAGCLASGAAEVIAAFKTELEFHGLTTEVNTKGTGCPGFCERGPIVMI-YPEG 84
           PC SV       A GA +V  A   E E   L  ++    T   G     P++     +G
Sbjct: 13  PCDSVAR-----AVGADQVAEALLREAERRQLPLDIQR--TSSRGLYWLEPLIECDSAQG 65

Query: 85  -ICYLKVKPEDVPEIVSHTIKEKKVVDRLLYEDPATGTRALRE-SDIPFYKNQQRNILSE 142
            + +  + P DVP ++             L  +P   + AL    +IP+ K QQR + + 
Sbjct: 66  RLGFGPITPHDVPSLLD-----------ALAGEPGGHSLALGPVEEIPYLKTQQRLLFAR 114

Query: 143 NLRLDSKSMDDYLAIGGYSALSKVLFQMTPEDVMGEIKKSNLRGRGGGGFPAWRKWEESR 202
                  S+DDY A GG++ L   +  +   +V+  +  S LRGRGG  FPA  KW   R
Sbjct: 115 AGITRPLSLDDYRANGGFAGLEAAV-ALDGAEVVAAVLDSGLRGRGGAAFPAGIKWRTVR 173

Query: 203 NAPDPIKYVIVNADEGDPGAFMDRALIEGNPHSILEGLIIGAYAVGAHEGFIYVRQEYPL 262
           +A    KYV+ NADEGD G F DR L+EG+P  ++EG+II   AVGA +G+IYVR EYP 
Sbjct: 174 DAGAGQKYVVCNADEGDSGTFADRMLMEGDPFLLIEGMIIAGLAVGADKGYIYVRSEYPD 233

Query: 263 AVENINLAIRQASERGFVGKDILGSGFDFTVKVHMGAGAFVCGESSALMTALEGRAGEPR 322
           A+  ++ A   A + G++G D+ GSG  F ++V +GAGA++CGE +AL+ ++EG+ G  R
Sbjct: 234 AIRVLDQAFAIARDAGYLGADVAGSGQAFDLEVRVGAGAYICGEETALLESIEGKRGIVR 293

Query: 323 PKYIHTAVKGVWDHPSVLNNVETWANVTQIITKGADWFTSYGTAGSTGTKIFSLVGKITN 382
            K    A++G++  P++++NV T A+V  I+ KGA ++  +G   S GT  F L G I  
Sbjct: 294 AKPPLPALQGLFGLPTLVHNVLTLASVPIILAKGAAFYRDFGMGRSLGTMPFQLAGNIRQ 353

Query: 383 TGLVEVPMGVTLRDIITKVGGGIPGGKKFKAVQTGGPSGGCIPEAMLDLPVDFDELTKAG 442
            GLVE   G+TLR+++   GGG   G+  KA Q GGP G  +P +  D P+D++     G
Sbjct: 354 GGLVERAFGLTLRELVEGYGGGTASGRPLKAAQVGGPLGAWVPPSHFDTPLDYEAFAAVG 413

Query: 443 SMMGSGGMIVMDEDTCMVDIARYFIDFLKDESCGKCTPCREGIRQMLAVLTRITVGKG-- 500
           +M+G GG++V D+   M  +AR+ + F  +ESCGKCTPCR G  + + V+ R+       
Sbjct: 414 AMLGHGGVVVADDTLNMASMARFALQFCAEESCGKCTPCRIGSTRGMEVVDRLIASSDIT 473

Query: 501 -KEGDIELLEELAESTG-AALCALGKSAPNPVLSTIRYFRDEY 541
            +     LL +L ++    +LCA+G     PV S ++YF  ++
Sbjct: 474 ERHDQALLLRDLCDTLQYGSLCAMGGMTAYPVASALKYFPADF 516


Lambda     K      H
   0.319    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 842
Number of extensions: 51
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 635
Length of database: 525
Length adjustment: 36
Effective length of query: 599
Effective length of database: 489
Effective search space:   292911
Effective search space used:   292911
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources