GapMind for catabolism of small carbon sources

 

Aligments for a candidate for paaJ2 in Pseudomonas putida KT2440

Align β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized)
to candidate PP_4636 PP_4636 acetyl-CoA acetyltransferase

Query= metacyc::MONOMER-15952
         (401 letters)



>lcl|FitnessBrowser__Putida:PP_4636 PP_4636 acetyl-CoA
           acetyltransferase
          Length = 392

 Score =  345 bits (886), Expect = 1e-99
 Identities = 202/402 (50%), Positives = 263/402 (65%), Gaps = 12/402 (2%)

Query: 1   MNEALIIDAVRTPIGRYAGALASVRADDLGAIPLKALIARHPQLDWSAVDDVIYGCANQA 60
           MN+ +I+ A RT IG + GALA+V A DLGA  +K L+ +   LD + VD+VI G    A
Sbjct: 1   MNDVVIVAATRTAIGSFQGALATVPAVDLGAAVIKQLL-KQTGLDPAQVDEVILGQVLTA 59

Query: 61  GEDNRNVARMAALLAGLPVSVPGTTLNRLCGSGLDAVGSAARALRCGEAGLMLAGGVESM 120
           G   +N AR AA+ AGLP SVP  TLN++CGSGL A+  AA+A+RCG+A +++AGG E+M
Sbjct: 60  GA-GQNPARQAAIKAGLPFSVPALTLNKVCGSGLKALHLAAQAIRCGDAEVVIAGGQENM 118

Query: 121 SRAPFVM--GKSEQAFGRSAEIFDTTIGWRFVNKLMQQGFGIDSMPETAENVAAQFNISR 178
           S AP+VM   ++ Q  G   ++ D+ I     +       GI     TAEN+  ++ +SR
Sbjct: 119 SLAPYVMPSARTGQRMGHG-QLIDSMITDGLWDAFNDYHMGI-----TAENLVDKYGLSR 172

Query: 179 ADQDAFALRSQHKAAAAIANGRLAKEIVAVEIAQRKGPAKIVEHDEHPRGDTTLEQLAKL 238
             QDAFA  SQ KA AAI  GR   EI  + + Q+KG  K+   DE PR DTT E LAKL
Sbjct: 173 EQQDAFAAESQRKAVAAIEAGRFDAEITPIVLPQKKGEPKVFARDEQPRPDTTAESLAKL 232

Query: 239 GTPFRQGGSVTAGNASGVNDGACALLLASSEAAQRHGLKARARVVGMATAGVEPRIMGIG 298
              F++ GSVTAGNAS +NDGA A+LL S+  A+  GL   A++   A+AGV+P IMGIG
Sbjct: 233 RPAFKKDGSVTAGNASSLNDGAAAVLLMSAAKAEALGLPVLAKIAAYASAGVDPAIMGIG 292

Query: 299 PVPATRKVLELTGLALADMDVIELNEAFAAQGLAVLRELGLADDDERVNPNGGAIALGHP 358
           PV AT++ L+  G  LA++D+IE NEAFAAQ LAV     LA D  RVN NGGAIALGHP
Sbjct: 293 PVSATQRCLDKAGWQLAELDLIEANEAFAAQALAVGN--ALAWDAARVNVNGGAIALGHP 350

Query: 359 LGMSGARLVTTALHELEERQGRYALCTMCIGVGQGIALIIER 400
           +G SG R++ T LHE+ +R  +  L T+CIG GQG+AL IER
Sbjct: 351 IGASGCRVLVTLLHEMIKRDVKKGLATLCIGGGQGVALAIER 392


Lambda     K      H
   0.319    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 398
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 401
Length of database: 392
Length adjustment: 31
Effective length of query: 370
Effective length of database: 361
Effective search space:   133570
Effective search space used:   133570
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory