GapMind for catabolism of small carbon sources

 

Finding step artQ for L-arginine catabolism in Pseudomonas putida KT2440

5 candidates for artQ: L-arginine ABC transporter, permease component 2 (ArtQ/HisQ/AotQ)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi PP_4485 histidine / lysine / arginine / ornithine ABC transporter - permease subunit ABC transporter for L-Arginine, permease component 1 (characterized) 91% 100% 416.4
hi PP_0281 Amino acid ABC transporter, permease protein Probable permease of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 67% 100% 318.2
med PP_3594 Amino acid ABC transporter, membrane protein Probable permease of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 39% 94% 156.8 Octopine transport system permease protein occQ, component of Octopine porter 35% 150.6
lo PP_0226 sulfur compound ABC transporter - permease subunit Probable permease of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 35% 97% 123.6 L-cystine transport system permease protein YecS 72% 325.5
lo PP_0656 putative Amino acid ABC transporter, permease protein ABC transporter for L-Arginine, permease component 2 (characterized) 34% 96% 123.2 Arginine transport system permease protein ArtQ 40% 160.6

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step artQ

Or cluster all characterized artQ proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory