Align Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (characterized)
to candidate PP_2588 PP_2588 Aminotransferase, class III
Query= reanno::BFirm:BPHYT_RS23155 (482 letters) >FitnessBrowser__Putida:PP_2588 Length = 460 Score = 553 bits (1425), Expect = e-162 Identities = 267/453 (58%), Positives = 336/453 (74%), Gaps = 3/453 (0%) Query: 29 QRSTAEYRALDAAHHIHPFSDMGSLNRSGSRVIVKAQGVYLWDSEGNKVIDGMAGLWCVN 88 QR T +Y+A DAAHHIH F D +LN G RVIV + ++LWDSEG + +DGM+GLWC Sbjct: 8 QRQTRDYQAADAAHHIHAFLDQKALNAEGPRVIVGGERLHLWDSEGKRYLDGMSGLWCTQ 67 Query: 89 VGYGRKELADAAYKQMQELPYYNTFFKTTHPPVIELSALLAELAPEAFNHFFYCNSGSEG 148 +GYGR++L AA QM +L YYN FF TTHP VIELS LL L P ++H Y NSGSE Sbjct: 68 LGYGRRDLTAAAATQMDQLAYYNMFFHTTHPAVIELSELLFSLLPGHYSHAIYTNSGSEA 127 Query: 149 NDTVLRIVHQYWATQGKHSKKFVISRKNGYHGSTIAGGTLGGMGYMHEQMPSKVENIVHI 208 N+ ++R V +YW G+ KK +I R NGYHGST+A LGGM +MH+ M + ++ HI Sbjct: 128 NEVLIRTVRRYWQVVGQPGKKIMIGRWNGYHGSTLAATALGGMKFMHD-MGGLIPDVAHI 186 Query: 209 DQPYFFGEAQGNLTPEEFALARAQQLEAKILEIGADNVAAFIGEPFQGAGGVIFPASTYW 268 D+PY++ E G LTP EF A QLE KILE+GA+NVA FI EPFQGAGG+IFP +YW Sbjct: 187 DEPYWYAEG-GELTPAEFGRRCALQLEEKILELGAENVAGFIAEPFQGAGGMIFPPESYW 245 Query: 269 PEIQRICRKYDILLVADEVIGGFGRTGEWFAHQHFGFEPDLITLAKGLTSGYVPMGAVGL 328 PEIQRICR+YD+LL ADEVIGGFGRTGEWFAH++FGFEPD +++AKGLTSGYVPMG + L Sbjct: 246 PEIQRICRQYDVLLCADEVIGGFGRTGEWFAHEYFGFEPDTLSIAKGLTSGYVPMGGLVL 305 Query: 329 HDRVAKAIIENGD-FNHGLTYSGHPVAAAVAVANLKLLRDEKIVDRVKNDTGPYFQKQLR 387 R+A+A++E G F HGLTYSGHPVAAAVA+ANLK LRDE IV +VK+DTGPY Q+ LR Sbjct: 306 SKRIAEALVERGGVFAHGLTYSGHPVAAAVAIANLKALRDEGIVRQVKDDTGPYLQRILR 365 Query: 388 ETFANHPIIGEISGTGLVAGLQLAQDPKARKRFANGGDVGTICRDFCFNGNLIMRATGDR 447 E FA+HP+IG++ G GLVA LQ A+ RKRFAN D+ CR F F +I+R+T R Sbjct: 366 EVFADHPLIGQVQGAGLVAALQFAEHKPTRKRFANENDLAWQCRTFGFEEGVIIRSTLGR 425 Query: 448 MLLSPPLVINKLEIDEIVSKAKKAIDATAQQLG 480 M+++P L+ N E+DE+V K + A+D TA+ +G Sbjct: 426 MIMAPALIANHSELDELVEKTRIAVDRTARLVG 458 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 641 Number of extensions: 30 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 482 Length of database: 460 Length adjustment: 33 Effective length of query: 449 Effective length of database: 427 Effective search space: 191723 Effective search space used: 191723 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory