Alignments for a candidate for puuA in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuA in Pseudomonas putida KT2440

Align Glutamate--putrescine ligase (EC 6.3.1.11) (characterized)
to candidate PP_3148 PP_3148 putative Glutamine synthetase

Query= reanno::MR1:200446
         (451 letters)



>FitnessBrowser__Putida:PP_3148
          Length = 452

 Score =  676 bits (1744), Expect = 0.0
 Identities = 324/450 (72%), Positives = 381/450 (84%), Gaps = 3/450 (0%)

Query: 2   NKLIAFLKERKITEVECVICDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVDD 61
           ++L  +LKE +ITEVECVI D+TGIARGKIAP  KFL E+GMRLPESVLLQTVTGD+VDD
Sbjct: 6   DQLSTWLKEHRITEVECVISDLTGIARGKIAPTAKFLHERGMRLPESVLLQTVTGDYVDD 65

Query: 62  DIYYSLLDDADIDFVCVPDENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVL 121
           DIYY+LLD ADID VC PD  AV+ +PW IE TA VIHD +D+ GNPIELSPRNVLKKVL
Sbjct: 66  DIYYNLLDAADIDMVCRPDPTAVYQIPWAIEPTAIVIHDTFDKQGNPIELSPRNVLKKVL 125

Query: 122 SLYDEKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSGRPEAGRQSFSIDAANEYDPLF 181
            LY +KGW+P++APEMEFYLT R +D DLPL+ P+GRSGR E+GRQSFSIDAANE+DPLF
Sbjct: 126 KLYADKGWQPIVAPEMEFYLTKRCEDPDLPLQVPLGRSGRAESGRQSFSIDAANEFDPLF 185

Query: 182 EDMYDWCEIQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVCA 241
           ED+YDWCEIQGLD+DTLIHEDGPAQMEINF HG+ L LADQ+ VFKRT+REAALKHNV A
Sbjct: 186 EDVYDWCEIQGLDLDTLIHEDGPAQMEINFRHGDALDLADQITVFKRTMREAALKHNVAA 245

Query: 242 TFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSALFLSYIAGLQKYIPEFLPLM 301
           TFMAKP+TDEPGSAMH+HQSV++  TGK IF NEDG+ S LFL +I GLQKYIP+ LP+ 
Sbjct: 246 TFMAKPITDEPGSAMHLHQSVVDIATGKPIFANEDGSMSQLFLHHIGGLQKYIPKLLPMF 305

Query: 302 APNANSFRRFLPGTSAPVNLEWGIENRTCGLRIPESSPQNRRIENRIPGADANCYLAFAA 361
           APN NSFRRFLP TSAPVN+EWG ENRT GLR+P SSP+  R+ENR+PGADAN YLA AA
Sbjct: 306 APNVNSFRRFLPDTSAPVNVEWGEENRTAGLRVPTSSPEAMRVENRLPGADANPYLAIAA 365

Query: 362 GLLCGYIGMVEGLKPSTPVQGKANESRSNNPHCLPLTLEEALVAMEESDACKEYLGESFT 421
            LLCGY+GMVE + PS PVQG+A E R+     LP+T+E+AL  ME+ +  ++YLG+ F 
Sbjct: 366 SLLCGYLGMVEQIDPSAPVQGRAYERRNLR---LPITIEDALQHMEDCETVQQYLGKQFV 422

Query: 422 TGFVAVKQAELENFRRVVSSWEREFLLLSV 451
            G+VAVK+AE EN++RV+SSWEREFL+LSV
Sbjct: 423 QGYVAVKRAEHENYKRVISSWEREFLMLSV 452


Lambda     K      H
   0.319    0.137    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 684
Number of extensions: 23
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 451
Length of database: 452
Length adjustment: 33
Effective length of query: 418
Effective length of database: 419
Effective search space:   175142
Effective search space used:   175142
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources