Alignments for a candidate for yveA in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for yveA in Pseudomonas putida KT2440

Align Aspartate-proton symporter; L-aspartate transporter (characterized)
to candidate PP_1259 PP_1259 Aspartate-proton symporter

Query= SwissProt::O07002
         (520 letters)



>FitnessBrowser__Putida:PP_1259
          Length = 537

 Score =  665 bits (1717), Expect = 0.0
 Identities = 302/508 (59%), Positives = 405/508 (79%), Gaps = 1/508 (0%)

Query: 5   GNFQKSMSLFDLILIGMGAIFGSAWLFAVSNVASKAGPSGAFSWILGGAIILLIGLVYAE 64
           G F+K +SL DL  IG+GAIFGS WLFA S+V++ AGP+G  SW LGG  +LL+G+VY E
Sbjct: 3   GKFKKQLSLLDLTFIGLGAIFGSGWLFAASHVSAIAGPAGILSWFLGGFAVLLLGIVYCE 62

Query: 65  LGAALPRTGGIIRYPVYSHGHLVGYLISFVTIVAYTSLISIEVTAVRQYVAYWFPGLTIK 124
           LGAALPR GG++RYPVYSHG L+GYL+ F+T++A++SLI+IEV A RQY A WFPGLT  
Sbjct: 63  LGAALPRAGGVVRYPVYSHGPLLGYLMGFITLIAFSSLIAIEVVASRQYAAAWFPGLTKA 122

Query: 125 GSDSPTISGWILQFALLCLFFLLNYWSVKTFAKANFIISIFKYIVPITIIIVLIFHFQPE 184
           GS  PT+ GW++QFALL LFF LNY SVKTFAKAN ++S+FK+IVP+ +I VL   F+PE
Sbjct: 123 GSSDPTVLGWLVQFALLGLFFFLNYRSVKTFAKANNLVSVFKFIVPLLVIGVLFTFFKPE 182

Query: 185 NLSVQGFAPFGFTGIQAAISTGGVMFAYLGLHPIVSVAGEVQNPKRNIPIALIICIIVST 244
           N  VQGFAPFG +G++ A+S GG++FAYLGL PI+SVA EV+NP+R IPIALI+ +++ST
Sbjct: 183 NFEVQGFAPFGLSGVEMAVSAGGIIFAYLGLTPIISVASEVKNPQRTIPIALILSVLLST 242

Query: 245 IIYTVLQVTFIGAIPTETLKHGWPAIGREFSLPFKDIAVMLGLGWLATLVILDAILSPGG 304
            IY +LQ+ F+G++PTE L +GW ++ +E +LP++DIA+ LG+GWLA LV+ DA++SP G
Sbjct: 243 AIYALLQLAFLGSVPTEMLANGWASVTKELALPYRDIALALGVGWLAYLVVADAVISPSG 302

Query: 305 NGNIFMNTTSRLVYAWARNGTLFGIFSKVNKDTGTPRASLWLSFALSIFWTLPFPSWNAL 364
            GNI+MN T R++Y WA+ GT F  F++++ ++G PR +LWL+F LS+FWTLPFPSW AL
Sbjct: 303 CGNIYMNATPRVIYGWAQTGTFFKYFTRIDAESGIPRPALWLTFGLSVFWTLPFPSWEAL 362

Query: 365 VNVCSVALILSYAIAPISSAALRVNAKDLNRPFYLKGMSIIGPLSFIFTAFIVYWSGWKT 424
           +NV S AL+LSYA+AP+S AALR NA  + RPF +KGM+++GPLSFI  A IVYWSGW T
Sbjct: 363 INVVSAALVLSYAVAPVSVAALRRNAPHMPRPFRVKGMTVLGPLSFIIAALIVYWSGWNT 422

Query: 425 VSWLLGSQLVMFLIYLCFSKYTPKEDVSLAQQLKSAWWLIGFYIMMLIFSYIGSFGHGLG 484
           VSWLL  Q+VMF++YL  S++ P + +SL QQ++S+ WLIGFY + ++ S++GSFG GLG
Sbjct: 423 VSWLLALQIVMFVLYLLCSRFVPTQHLSLGQQVRSSAWLIGFYALTILLSWLGSFG-GLG 481

Query: 485 IISNPVDLILVAIGSLAIYYWAKYTGLP 512
           +I +P D ++VA  +L IYYW   TG+P
Sbjct: 482 VIGHPFDTVVVAACALGIYYWGAATGVP 509


Lambda     K      H
   0.328    0.143    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1022
Number of extensions: 44
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 537
Length adjustment: 35
Effective length of query: 485
Effective length of database: 502
Effective search space:   243470
Effective search space used:   243470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources