GapMind for catabolism of small carbon sources

 

Aligments for a candidate for SMc04256 in Pseudomonas putida KT2440

Align ABC transporter for D-Cellobiose and D-Salicin, ATPase component (characterized)
to candidate PP_1018 PP_1018 mannose/glucose ABC transporter - ATP binding subunit

Query= reanno::Smeli:SMc04256
         (361 letters)



>lcl|FitnessBrowser__Putida:PP_1018 PP_1018 mannose/glucose ABC
           transporter - ATP binding subunit
          Length = 384

 Score =  327 bits (839), Expect = 2e-94
 Identities = 175/366 (47%), Positives = 244/366 (66%), Gaps = 6/366 (1%)

Query: 1   MTSVSVRDLSLNFGA--VTVLDRLNLDIDHGEFLVLLGSSGCGKSTLLNCIAGLLDVSDG 58
           M ++ +R+++  +G+     L  + L I  GEFL+L+G SGCGKSTL+NCIAGL  ++ G
Sbjct: 1   MATLELRNVNKTYGSGLPDTLKDIQLSIKDGEFLILVGPSGCGKSTLMNCIAGLEQITGG 60

Query: 59  QIFIKDRNVTWEEPKDRGIGMVFQSYALYPQMTVEKNLSFGLKVAKIPPAEIEKRVKRAS 118
            I I +++V+   PKDR I MVFQSYALYP M+V +N+ FGLK+ K+P A I++ V R +
Sbjct: 61  AILIDEQDVSGMSPKDRDIAMVFQSYALYPTMSVRENIEFGLKIRKLPQAAIDEEVARVA 120

Query: 119 EILQIQPLLKRKPSELSGGQRQRVAIGRALVRDVDVFLFDEPLSNLDAKLRSELRVEIKR 178
           ++LQI+ LL RKP++LSGGQ+QRVA+GRAL R   ++LFDEPLSNLDAKLR E+R E+K 
Sbjct: 121 KLLQIEHLLARKPAQLSGGQQQRVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTEMKL 180

Query: 179 LHQSLKNTMIYVTHDQIEALTLADRIAVMKSGVIQQLADPMTIYNAPENLFVAGFIGSPS 238
           +HQ LK T +YVTHDQIEA+TL D++AVMK G+IQQ   P  IYN P N FVA FIGSP 
Sbjct: 181 MHQRLKTTTVYVTHDQIEAMTLGDKVAVMKDGIIQQFGTPQQIYNDPANQFVASFIGSPP 240

Query: 239 MNFFRGEVEPKDGR--SFVRAGGIAFDVTAYPAHTRLQPGQKVVLGLRPEHVKVDEA-RD 295
           MNF    +  +DGR  + + +G    ++    A   L+ G++++LG+RPE + +  A  +
Sbjct: 241 MNFIPVRLARQDGRLLALLDSGQARCELPLGEAADALE-GREIILGIRPEQIALGAADGN 299

Query: 296 GEPTHQAVVDIEEPMGADNLLWLTFAGQSMSVRIAGQRRYPPGSTVRLSFDMGVASIFDA 355
           G P  +A V + EP G D L+++T     +  R+A       G T+ L FD     +FDA
Sbjct: 300 GLPAIRAEVQVTEPTGPDLLVFVTLNQTKVCCRLAPDVACRVGDTLNLQFDPARVLLFDA 359

Query: 356 ESENRL 361
            +  RL
Sbjct: 360 ANGERL 365


Lambda     K      H
   0.320    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 395
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 384
Length adjustment: 30
Effective length of query: 331
Effective length of database: 354
Effective search space:   117174
Effective search space used:   117174
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory