GapMind for catabolism of small carbon sources

 

Alignments for a candidate for SMc04256 in Pseudomonas putida KT2440

Align ABC transporter for D-Cellobiose and D-Salicin, ATPase component (characterized)
to candidate PP_1722 PP_1722 ABC transporter, ATP-binding protein

Query= reanno::Smeli:SMc04256
         (361 letters)



>FitnessBrowser__Putida:PP_1722
          Length = 329

 Score =  227 bits (578), Expect = 4e-64
 Identities = 117/236 (49%), Positives = 160/236 (67%)

Query: 1   MTSVSVRDLSLNFGAVTVLDRLNLDIDHGEFLVLLGSSGCGKSTLLNCIAGLLDVSDGQI 60
           M+ VSV+ L  ++    V +R++  I+ GEF+ LLG SGCGKSTLL CIAGL  V  GQI
Sbjct: 1   MSFVSVQKLQKSYAGSPVFERIDCHIERGEFVTLLGPSGCGKSTLLRCIAGLTSVDSGQI 60

Query: 61  FIKDRNVTWEEPKDRGIGMVFQSYALYPQMTVEKNLSFGLKVAKIPPAEIEKRVKRASEI 120
            +   ++    P+ RGIGMVFQSYAL+P MTVE+N++FGL++ K+   E + RV+   E+
Sbjct: 61  LLDGHDIVPLSPQKRGIGMVFQSYALFPNMTVEQNVAFGLRMQKVKADESQLRVREVLEL 120

Query: 121 LQIQPLLKRKPSELSGGQRQRVAIGRALVRDVDVFLFDEPLSNLDAKLRSELRVEIKRLH 180
           +++     R P +LSGGQ QRVA+ R+LV    + L DEPLS LDA++R  LR +I+ + 
Sbjct: 121 VELGKFAGRYPHQLSGGQCQRVALARSLVTRPRLLLLDEPLSALDARIRKHLREQIRAIQ 180

Query: 181 QSLKNTMIYVTHDQIEALTLADRIAVMKSGVIQQLADPMTIYNAPENLFVAGFIGS 236
           + L  T I+VTHDQ EALT++DRI +M  G I Q  D  T+Y AP +LF AGFIG+
Sbjct: 181 RELGLTTIFVTHDQEEALTMSDRIFLMNQGRIVQSGDAETLYTAPVDLFAAGFIGN 236



 Score = 24.3 bits (51), Expect = 0.005
 Identities = 12/36 (33%), Positives = 17/36 (47%)

Query: 259 GIAFDVTAYPAHTRLQPGQKVVLGLRPEHVKVDEAR 294
           GI     +Y     +   Q V  GLR + VK DE++
Sbjct: 76  GIGMVFQSYALFPNMTVEQNVAFGLRMQKVKADESQ 111


Lambda     K      H
   0.320    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 302
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 361
Length of database: 329
Length adjustment: 29
Effective length of query: 332
Effective length of database: 300
Effective search space:    99600
Effective search space used:    99600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory