Alignments for a candidate for acn in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for acn in Pseudomonas putida KT2440

Align 2-methylcitrate dehydratase; 2-MC dehydratase; Aconitate hydratase; ACN; Aconitase; EC 4.2.1.79; EC 4.2.1.3 (characterized)
to candidate PP_2338 PP_2338 2-methylcitrate dehydratase

Query= SwissProt::Q937N6
         (484 letters)



>FitnessBrowser__Putida:PP_2338
          Length = 494

 Score =  576 bits (1485), Expect = e-169
 Identities = 294/497 (59%), Positives = 362/497 (72%), Gaps = 16/497 (3%)

Query: 1   MSTDTPSAQRPDPDQVLVDIVDYVTRYEIKSGLAYDTARNCLIDTLGCGLEALSYPACTK 60
           MS +     RPD D+VL  + DY   Y + S  A DTARNCL+DTLGCGL AL +P CTK
Sbjct: 1   MSANVDLNDRPDYDRVLQTLADYALGYRVDSREALDTARNCLMDTLGCGLLALRFPECTK 60

Query: 61  LLGPVVPGTIVPNGARVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGG 120
           LLGP V GT+VPNGARVPGT ++LDPV+AA++IG  +RWLD+NDTWLAAEW HPSDNLGG
Sbjct: 61  LLGPQVEGTLVPNGARVPGTSYRLDPVKAAWDIGCTVRWLDYNDTWLAAEWAHPSDNLGG 120

Query: 121 ILATADWISRNHIAAGRKPLTMKAVLAAMIKAHEIQGCIALENSFNQVGLDHVVLVKVAS 180
           ILA AD +S+  +AAG KPL M+ VL AM+ AHEIQG +ALEN+FN+VGLDHV+LVKVAS
Sbjct: 121 ILAVADHLSQKRVAAGEKPLLMRDVLEAMVMAHEIQGVLALENAFNRVGLDHVILVKVAS 180

Query: 181 TAVVAQMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNAGSRKSWAHGDATSRAVRLALIA 240
           TAV A+++G  R+++L+A+S A+VDGQ+LRTYRHAPNAGSRKSWA GDA+SR VRLA IA
Sbjct: 181 TAVCARLMGANREQMLSALSHAFVDGQALRTYRHAPNAGSRKSWAAGDASSRGVRLADIA 240

Query: 241 RTGEMGYPSVLTAKTWGFYDVLFK-----------GQ-PFRFQRPYGTYVMENILLKISY 288
             GEMG P VLTA  WGFYDV F            GQ   R  +  G+YVMEN+L K+S+
Sbjct: 241 LRGEMGVPGVLTASQWGFYDVSFSHTNKDLALKPAGQYELRLPQALGSYVMENVLFKVSF 300

Query: 289 PAEFHAQTAVEAAMQLHGALALAGRSAADIAAITIRTHEACLRIIDKLGPLSNPADRDHC 348
           PAEFHAQTA EAA+ LH    L      ++  I I T E+ +RII K GPL+N ADRDHC
Sbjct: 301 PAEFHAQTACEAAVTLH---PLVRNRLHEVDRIVITTQESAIRIISKSGPLANAADRDHC 357

Query: 349 IQYMVAVPLLFGRLTAADYEDRIAAD-PRIDALRGRITCVEDPALTRDYHDPAKRSIANA 407
           +QYMVAVPL+FG L A  YED   A+ P ID LR ++  VEDP  +R+Y +P KRSIANA
Sbjct: 358 LQYMVAVPLIFGHLVAEHYEDAFHANHPSIDRLREKMEVVEDPRFSREYLEPGKRSIANA 417

Query: 408 LTVTLADGTVLDEVLVEYPLGHKRRRAEGIPLLVEKFRTNLARRFPPKQQQAILAASLDQ 467
           L V   DG+  ++V+VEYP+GH+RRR EGIPLL  KFR NLA RF  ++ Q +     DQ
Sbjct: 418 LQVFFKDGSSTEQVVVEYPIGHRRRRGEGIPLLEAKFRANLATRFARQRCQQVFELCKDQ 477

Query: 468 ARLEAMPVNEYLDMYAI 484
            +LE   V+ ++D++ I
Sbjct: 478 QKLEGTAVHRFVDLFVI 494


Lambda     K      H
   0.322    0.136    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 643
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 484
Length of database: 494
Length adjustment: 34
Effective length of query: 450
Effective length of database: 460
Effective search space:   207000
Effective search space used:   207000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources