GapMind for catabolism of small carbon sources

 

L-citrulline catabolism in Pseudomonas putida KT2440

Best path

AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, aruF, aruG, astC, astD, astE

Also see fitness data for the top candidates

Rules

Overview: Citrulline can be catabolized via ornithine carbamoyltransferase in reverse (PMID:3129535). Genetic evidence suggests that some bacteria use a putative citrullinase (EC 3.5.1.20) to consume citrulline.

51 steps (41 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
AO353_03055 ABC transporter for L-Citrulline, periplasmic substrate-binding component PP_4486 PP_0282
AO353_03050 ABC transporter for L-Citrulline, permease component 1 PP_4485 PP_0281
AO353_03045 ABC transporter for L-Citrulline, permease component 2 PP_4484 PP_0280
AO353_03040 ABC transporter for L-Citrulline, ATPase component PP_4483 PP_0283
arcB ornithine carbamoyltransferase PP_1000 PP_1079
arcC carbamate kinase PP_0999
aruF ornithine/arginine N-succinyltransferase subunit AruAI (AruF) PP_4480 PP_4479
aruG ornithine/arginine N-succinyltransferase subunit AruAII (AruG) PP_4479 PP_4480
astC succinylornithine transaminase PP_4481 PP_0372
astD succinylglutamate semialdehyde dehydrogenase PP_4478 PP_5278
astE succinylglutamate desuccinylase PP_4475
Alternative steps:
atoB acetyl-CoA C-acetyltransferase PP_2215 PP_4636
citrullinase putative citrullinase PP_0382
davD glutarate semialdehyde dehydrogenase PP_0213 PP_4422
davT 5-aminovalerate aminotransferase PP_0214 PP_4108
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase PP_2217 PP_3284
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase PP_2136 PP_3755
gabD succinate semialdehyde dehydrogenase PP_0213 PP_3151
gabT gamma-aminobutyrate transaminase PP_5182 PP_2180
gcdG succinyl-CoA:glutarate CoA-transferase PP_0159 PP_3001
gcdH glutaryl-CoA dehydrogenase PP_0158 PP_4064
glaH glutarate 2-hydroxylase, succinate-releasing (GlaH or CsiD) PP_2909
lhgD L-2-hydroxyglutarate dehydrogenase or oxidase (LhgD or LhgO) PP_2910
ocd ornithine cyclodeaminase PP_3533 PP_4431
odc L-ornithine decarboxylase PP_0864 PP_4140
oraE D-ornithine 4,5-aminomutase, beta (E) subunit
oraS D-ornithine 4,5-aminomutase, alpha (S) subunit
ord 2,4-diaminopentanoate dehydrogenase
orr ornithine racemase
ortA 2-amino-4-oxopentanoate thiolase, alpha subunit
ortB 2-amino-4-oxopentanoate thiolase, beta subunit
patA putrescine aminotransferase (PatA/SpuC) PP_5182 PP_2180
patD gamma-aminobutyraldehyde dehydrogenase PP_1481 PP_2801
prdA D-proline reductase, prdA component
prdB D-proline reductase, prdB component
prdC D-proline reductase, electron transfer component PrdC
prdF proline racemase PP_1258
PRO3 pyrroline-5-carboxylate reductase PP_5095 PP_3778
PS417_17590 ABC transporter for L-Citrulline, periplasmic substrate-binding component PP_4486 PP_0282
PS417_17595 ABC transporter for L-Citrulline, permease component 1 PP_4485 PP_0281
PS417_17600 ABC transporter for L-Citrulline, permease component 2 PP_4484 PP_0280
PS417_17605 ABC transporter for L-Citrulline, ATPase component PP_4483 PP_0283
puo putrescine oxidase
put1 proline dehydrogenase PP_4947
putA L-glutamate 5-semialdeyde dehydrogenase PP_4947 PP_2694
puuA glutamate-putrescine ligase PP_5184 PP_5299
puuB gamma-glutamylputrescine oxidase PP_2448 PP_3146
puuC gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase PP_5278 PP_2589
puuD gamma-glutamyl-gamma-aminobutyrate hydrolase PP_3598 PP_2179
rocA 1-pyrroline-5-carboxylate dehydrogenase PP_4947 PP_2694
rocD ornithine aminotransferase PP_0214 PP_4223

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory