Alignments for a candidate for astD in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for astD in Pseudomonas putida KT2440

Align Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) (characterized)
to candidate PP_4478 PP_4478 N-succinylglutamate 5-semialdehyde dehydrogenase

Query= reanno::pseudo13_GW456_L13:PfGW456L13_1974
         (488 letters)



>FitnessBrowser__Putida:PP_4478
          Length = 487

 Score =  814 bits (2102), Expect = 0.0
 Identities = 406/487 (83%), Positives = 437/487 (89%)

Query: 1   MKSLYIAGEWLAGGGEAFESLNPVTQQVLWSGVGATAGQVESAVQAARQAFPDWARRTLE 60
           M + YIAG W AG GE  +SLNPVTQ V+W G GA A QV++AVQAARQAFP WA+ +LE
Sbjct: 1   MTTHYIAGNWQAGQGETLQSLNPVTQAVIWQGQGADASQVDAAVQAARQAFPAWAQLSLE 60

Query: 61  ERISVLEAFAAALKNHADELAHTIGEETGKPLWEAATEVTSMVNKIAISVQSYRERTGEK 120
            RI VLE FAA LK HA+ +A  IGEETGKPLWE+ATEVTSM+NK+AISVQSYRERTGEK
Sbjct: 61  ARIDVLEKFAAQLKVHAEAMAQCIGEETGKPLWESATEVTSMINKVAISVQSYRERTGEK 120

Query: 121 SGPLGDATAVLRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGNSVLFKPSELTPKVAEL 180
           SGPL DATAVLRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGN V+FKPSELTPKVAEL
Sbjct: 121 SGPLADATAVLRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGNCVVFKPSELTPKVAEL 180

Query: 181 TVKCWIEAGLPAGVLNLLQGARETGIALAANPGIDGLFFTGSSRTGNHLHQQFAGRPDKI 240
           TV CWI AGLPAGVLNL+QGARETG+ALAANPGIDGLFFTGSSRTGN LHQQFAGRPDKI
Sbjct: 181 TVNCWIAAGLPAGVLNLVQGARETGVALAANPGIDGLFFTGSSRTGNLLHQQFAGRPDKI 240

Query: 241 LALEMGGNNPLVVDQVADLDAAVYTIIQSAFISAGQRCTCARRLLVPQGAWGDSLLARLV 300
           LALEMGGNNPLVVD+V DLDAAVYTIIQSAFISAGQRCTCARRLLVPQGAWGD+L+ARLV
Sbjct: 241 LALEMGGNNPLVVDEVKDLDAAVYTIIQSAFISAGQRCTCARRLLVPQGAWGDALIARLV 300

Query: 301 AVSSTLSVGAFDQQPAPFMGSVVSLGAAKALMDAQEHLLANGAVALLEMTQPQAQSALLT 360
            V  T++VGAFD+QPAPFMGSV+SL AA+AL+ AQ  L A G V LLEMTQPQA +ALLT
Sbjct: 301 EVCKTITVGAFDEQPAPFMGSVISLQAARALLAAQVELAAKGGVKLLEMTQPQADAALLT 360

Query: 361 PGILDVSAVADRPDEELFGPLLQVIRYADFEAAIAEANDTAYGLAAGLLSDSEARYQQFW 420
           PGI+DV+AVADRPDEE FGPLLQVIRYADF+AAI EAN+T YGLAAGLLSDS ARYQ FW
Sbjct: 361 PGIVDVTAVADRPDEEFFGPLLQVIRYADFDAAIDEANNTQYGLAAGLLSDSRARYQYFW 420

Query: 421 LESRAGIVNWNKQLTGAASSAPFGGVGASGNHRASAYYAADYCAYPVASLETPSLVLPSA 480
           L SRAGIVNWNKQLTGAASSAPFGGVGASGNHRASAYYAADYCAYPVASLET SL LP+ 
Sbjct: 421 LRSRAGIVNWNKQLTGAASSAPFGGVGASGNHRASAYYAADYCAYPVASLETASLALPAT 480

Query: 481 LTPGVKM 487
           LTPGV +
Sbjct: 481 LTPGVTL 487


Lambda     K      H
   0.316    0.132    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 802
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 488
Length of database: 487
Length adjustment: 34
Effective length of query: 454
Effective length of database: 453
Effective search space:   205662
Effective search space used:   205662
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate PP_4478 PP_4478 (N-succinylglutamate 5-semialdehyde dehydrogenase)
to HMM TIGR03240 (astD: succinylglutamate-semialdehyde dehydrogenase (EC 1.2.1.71))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03240.hmm
# target sequence database:        /tmp/gapView.9610.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03240  [M=484]
Accession:   TIGR03240
Description: arg_catab_astD: succinylglutamate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                           Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                           -----------
   1.8e-266  870.3   4.0   2.1e-266  870.1   4.0    1.0  1  lcl|FitnessBrowser__Putida:PP_4478  PP_4478 N-succinylglutamate 5-se


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Putida:PP_4478  PP_4478 N-succinylglutamate 5-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  870.1   4.0  2.1e-266  2.1e-266       1     483 [.       4     486 ..       4     487 .] 1.00

  Alignments for each domain:
  == domain 1  score: 870.1 bits;  conditional E-value: 2.1e-266
                           TIGR03240   1 lfidGkwraGqGeslesldpvtqevlwqgkaasaaqvekavkaarkafpawarlsleeriavvkrfaelleeeke 75 
                                         ++i G+w+aGqGe+l+sl+pvtq+v+wqg++a+a+qv++av+aar+afpawa+lsle+ri+v+++fa++l+ ++e
  lcl|FitnessBrowser__Putida:PP_4478   4 HYIAGNWQAGQGETLQSLNPVTQAVIWQGQGADASQVDAAVQAARQAFPAWAQLSLEARIDVLEKFAAQLKVHAE 78 
                                         69************************************************************************* PP

                           TIGR03240  76 elaeviaketgkplweartevasmvakvaisikayeertGekeseladakavlrhrphGvlavfGpynfpGhlpn 150
                                         ++a++i++etgkplwe++tev+sm++kvais+++y+ertGek+ +lada+avlrh+phGv+avfGpynfpGhlpn
  lcl|FitnessBrowser__Putida:PP_4478  79 AMAQCIGEETGKPLWESATEVTSMINKVAISVQSYRERTGEKSGPLADATAVLRHKPHGVVAVFGPYNFPGHLPN 153
                                         *************************************************************************** PP

                           TIGR03240 151 GhivpallaGntvvfkpseltplvaeetvklwekaGlpaGvlnlvqGaretGkalaaeedidGllftGssntGal 225
                                         GhivpallaGn+vvfkpseltp+vae tv++w +aGlpaGvlnlvqGaretG+alaa+++idGl+ftGss+tG+l
  lcl|FitnessBrowser__Putida:PP_4478 154 GHIVPALLAGNCVVFKPSELTPKVAELTVNCWIAAGLPAGVLNLVQGARETGVALAANPGIDGLFFTGSSRTGNL 228
                                         *************************************************************************** PP

                           TIGR03240 226 lhrqlagrpekilalelGGnnplvveevkdidaavhlivqsafisaGqrctcarrllvkdgaeGdallerlveva 300
                                         lh+q+agrp+kilale+GGnnplvv+evkd+daav++i+qsafisaGqrctcarrllv++ga+Gdal++rlvev+
  lcl|FitnessBrowser__Putida:PP_4478 229 LHQQFAGRPDKILALEMGGNNPLVVDEVKDLDAAVYTIIQSAFISAGQRCTCARRLLVPQGAWGDALIARLVEVC 303
                                         *************************************************************************** PP

                           TIGR03240 301 erltvgkydaepqpflGavisekaakellaaqekllalggksllelkqleeeaalltpgiidvtevaevpdeeyf 375
                                         +++tvg++d++p+pf+G+vis +aa++llaaq +l+a+gg +lle++q +++aalltpgi+dvt+va++pdee+f
  lcl|FitnessBrowser__Putida:PP_4478 304 KTITVGAFDEQPAPFMGSVISLQAARALLAAQVELAAKGGVKLLEMTQPQADAALLTPGIVDVTAVADRPDEEFF 378
                                         *************************************************************************** PP

                           TIGR03240 376 gpllkvlrykdfdealaeanntrfGlaaGllsddrelydkflleiraGivnwnkpltGassaapfGGiGasGnhr 450
                                         gpll+v+ry+dfd+a++eannt++GlaaGllsd+r++y++f+l++raGivnwnk+ltGa+s+apfGG+GasGnhr
  lcl|FitnessBrowser__Putida:PP_4478 379 GPLLQVIRYADFDAAIDEANNTQYGLAAGLLSDSRARYQYFWLRSRAGIVNWNKQLTGAASSAPFGGVGASGNHR 453
                                         *************************************************************************** PP

                           TIGR03240 451 psayyaadycaypvasleadslalpatlspGlk 483
                                         +sayyaadycaypvasle++slalpatl+pG++
  lcl|FitnessBrowser__Putida:PP_4478 454 ASAYYAADYCAYPVASLETASLALPATLTPGVT 486
                                         *******************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (484 nodes)
Target sequences:                          1  (487 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 11.87
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources