Aligments for a candidate for acs in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Aligments for a candidate for acs in Pseudomonas putida KT2440

Align isobutanoate/2-methylbutanoate--CoA ligase (EC 6.2.1.1) (characterized)
to candidate PP_4063 PP_4063 putative Long-chain-fatty-acid-CoA ligase

Query= metacyc::MONOMER-20125
         (556 letters)



>lcl|FitnessBrowser__Putida:PP_4063 PP_4063 putative
           Long-chain-fatty-acid-CoA ligase
          Length = 560

 Score =  164 bits (415), Expect = 8e-45
 Identities = 156/563 (27%), Positives = 243/563 (43%), Gaps = 64/563 (11%)

Query: 24  ATVYGDCTSVV----YDAVSYTWSQTHRRCLCLASSIASLGIENGHVVSVLAPNVPQMYE 79
           ATV   C S      +  + Y+W Q   +    A ++ +LG+  G  V + +PN  Q   
Sbjct: 26  ATVARCCDSEALVSRHQGLRYSWRQLAEQVEIYARALIALGVNTGDRVGIWSPNCAQWCI 85

Query: 80  LHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHLSRDLILEAIALFPKQAPVP 139
           L  A    GAIL  +N       +  +L  S  + +        +  EA         V 
Sbjct: 86  LQLASAKVGAILVNINPAYRVGELEYVLRQSGCRWL--------VCAEAFKTSDYHTMVQ 137

Query: 140 RLVFMADESESGNSSELGKEFFCSYKDLIDRGDPDFKWVMPKSEW--------------- 184
            LV    E  S    EL  E     + +I          +P   +               
Sbjct: 138 ELV---PELASAAPGELASECLPELRGVISLAANPPAGFLPWHAFAERAGQTSVEACTAR 194

Query: 185 -------DPMILNYTSGTTSSPKGVVHCHRGIF---IMTVDSLIDWGVPKQPVYLWTLPM 234
                   P+ + YTSGTT +PKG    H  I     M  +SL   G+  +   +  +P+
Sbjct: 195 QQSLQFDQPVNIQYTSGTTGAPKGATLSHYNILNNGFMVGESL---GLTARDRMVIPVPL 251

Query: 235 FHANGWSYP-WGMAAVGGTNICLRK-FDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGS 292
           +H  G      G    G T I     FD+E+    +     T + G P +   + + P  
Sbjct: 252 YHCFGMVMANLGCITHGSTMIYPNDAFDAELTLRAVAEERATILYGVPTMFIAMLDHPSR 311

Query: 293 E--PLKTTVQIMTAGAPPPSAVLFRT-ESLGFA-VSHGYGLTETAGLVVSCAWKKEWNHL 348
               L T    + AGA  P  V+ R  + +  A V   YG+TET+ + +      +   L
Sbjct: 312 AHMDLSTLRSGIMAGATCPIEVMRRVIDQMHMAEVQIAYGMTETSPVSLQTGPDDDLE-L 370

Query: 349 PATERARLKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEG 408
             T   R        T  Q +  +VD   G  V R    +GE+  RG SVMLGY  +P+ 
Sbjct: 371 RVTTVGR--------TQPQLENKLVD-ADGCIVPR--GEIGELCTRGYSVMLGYWDNPQA 419

Query: 409 TAKSMTADGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEA 468
           TA ++   GW ++GD+ VM   GY+ I  R+KD+II GGEN+   E+E   Y+HP + +A
Sbjct: 420 TADAIDPAGWMHSGDLAVMDEQGYVRIVGRNKDMIIRGGENIYPRELEEFFYTHPAVADA 479

Query: 469 AVVARPDEFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTS 528
            V+  P   +GE   A++ L  G     T +E+  +C++++  + VP+ + F +E P T 
Sbjct: 480 QVIGIPCSRYGEEIVAWIKLHPG--HSATVEELQGWCKARIAHFKVPRYIRFVDEYPMTV 537

Query: 529 TGKVQKFILRDMA-RGMGSATAG 550
           TGKVQKF +R+++   + +A+AG
Sbjct: 538 TGKVQKFRMREISVAEIAAASAG 560


Lambda     K      H
   0.319    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 752
Number of extensions: 44
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 556
Length of database: 560
Length adjustment: 36
Effective length of query: 520
Effective length of database: 524
Effective search space:   272480
Effective search space used:   272480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources