Align Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 (characterized)
to candidate PP_2674 PP_2674 quinoprotein ethanol dehydrogenase
Query= SwissProt::O05542 (757 letters) >FitnessBrowser__Putida:PP_2674 Length = 631 Score = 333 bits (853), Expect = 2e-95 Identities = 220/623 (35%), Positives = 314/623 (50%), Gaps = 63/623 (10%) Query: 6 LTPIKVTKKRLLSCAAALAFSAAVPVAFAQEDTGTAITSSDNGGH---PGDWLSYGRSYS 62 L+P+ ++ + LL + + A + G +T D D L YG + Sbjct: 9 LSPLALSVRVLLMAGSLALGNVATAASTPAAPAGKNVTWEDIANDHLTTQDVLQYGMGTN 68 Query: 63 EQRYSPLDQINTENVGKLKLAWHYDL--DTNRGQEGTPLIVNGVMYATTNWSKMKALDAA 120 QR+SPL Q+N +NV KL AW Y + RGQE ++ +GV+Y T ++S++ ALDA Sbjct: 69 AQRWSPLAQVNDKNVFKLTPAWSYSFGDEKQRGQESQAIVSDGVVYVTGSYSRVFALDAK 128 Query: 121 TGKLLWSYDPKVPGNIADRGCCDTVSRGAAYWNGKVYFGTFDGRLIALDAKTGKLVWSVY 180 TGK LW+Y+ ++P NI R CCD V+RGAA + K+YFGT D R+IALD +TGK+VW+ Sbjct: 129 TGKRLWTYNHRLPDNI--RPCCDVVNRGAAIYGDKIYFGTLDARVIALDKRTGKVVWN-- 184 Query: 181 TIPKEAQLG-HQRSYTVDGAPRIAK----GKVLI--GNGGAEFGARGFVSAFDAETGKLD 233 + G H YT+ GAP + K GKVL+ G+ G EFG G + A D +TG+ Sbjct: 185 -----KKFGDHSAGYTMTGAPVLIKDKTSGKVLLIHGSSGDEFGVVGQLFARDPDTGEEV 239 Query: 234 WRFFTVPNPENKPDGAASDDILMSKAYPTW--------GKNGAWKQQGGGGTVWDSLVYD 285 W V + +G S KA P+W GK AW GGG W S +D Sbjct: 240 WMRPFVEGHMGRLNGKDSTPTGDVKA-PSWPDDPTTETGKVEAWSH--GGGAPWQSASFD 296 Query: 286 PVTDLVYLGVGNGSPWNYKFRSEGKG-----DNLFLGSIVAINPDTGKYVWHFQETPMDE 340 P T+ + +G GN PWN R+ G D+L+ V ++P TG+ W +Q TP D Sbjct: 297 PETNTIIVGAGNPGPWNTWARTSKDGNPHDFDSLYTSGQVGVDPSTGEVKWFYQHTPNDA 356 Query: 341 WDYTSVQQIMTLDMP-VNGEMRHVIVHAPKNGFFYIIDAKTGKFITGKPYTYE-NWANGL 398 WD++ +++ D NG + HA +NGFFY++D GK P+ WA+ + Sbjct: 357 WDFSGNNELVLFDYKDKNGNVVKATAHADRNGFFYVVDRNNGKLQNAFPFVDNITWASHI 416 Query: 399 DPVTGRPNYVPDAL------WTLTGKPWLGIPGELGGHNFAAMAYSPKTKLVYIPAQQIP 452 D TGRP P GKP P LGG N+ MAYS T L YIP Q Sbjct: 417 DLKTGRPVENPGQRPAKPLPGETKGKPVEVSPPFLGGKNWNPMAYSQDTGLFYIPGNQWK 476 Query: 453 LLYDGQKGGFKAYHDAWNLGLDMNKIGLFDDNDPEHVAAKKDFLKVLKGWTVAWDPEKMA 512 Y ++ +K +G + + ++DD HV + A DP Sbjct: 477 EEYWTEEVNYKKGSAYLGMGFRIKR--MYDD----HVGTLR-----------AMDPTTGK 519 Query: 513 PAFTINHKGPWNGGLLATAGNVIFQGLANGEFHAYDATNGNDLYSFPAQSAIIAPPVTYT 572 + P G+LAT GN++F G +G F A+DA G +L+ F S I++PP+T+ Sbjct: 520 LVWEHKEHLPLWAGVLATKGNLVFTGTGDGFFKAFDAKTGKELWKFQTGSGIVSPPITWE 579 Query: 573 ANGKQYVAVEVGWGGIYPFLYGG 595 +G+QY+ V VG+GG P L+GG Sbjct: 580 QDGEQYIGVTVGYGGAVP-LWGG 601 Lambda K H 0.317 0.136 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2011 Number of extensions: 184 Number of successful extensions: 24 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 757 Length of database: 631 Length adjustment: 39 Effective length of query: 718 Effective length of database: 592 Effective search space: 425056 Effective search space used: 425056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory