Align Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 (characterized)
to candidate PP_2679 PP_2679 quinoprotein ethanol dehydrogenase
Query= SwissProt::P28036 (738 letters) >FitnessBrowser__Putida:PP_2679 Length = 595 Score = 344 bits (883), Expect = 7e-99 Identities = 211/587 (35%), Positives = 301/587 (51%), Gaps = 48/587 (8%) Query: 21 ICAALISGYATMASADDGQGATGEAIIHADDHPGNWMTYGRTYSDQRYSPLDQINRSNVG 80 + A L++G A A + E I+ +P +T G QRYSPLD +N +NV Sbjct: 16 LSAMLLAGAAHAA-------VSNEEILQDPKNPQQIVTNGLGVQGQRYSPLDLLNVNNVK 68 Query: 81 NLKLAWYLDL--DTNRGQEGTPLVIDGVMYATTNWSMMKAVDAATGKLLWSYDPRVPGNI 138 L+ W + RGQ+ PL+ DGVMY T ++S + AVDA TGK LW YD R+P +I Sbjct: 69 ELRPVWAFSFGGEKQRGQQAQPLIKDGVMYLTGSYSRVFAVDARTGKKLWQYDARLPDDI 128 Query: 139 ADKGCCDTVNRGAAYWNGKVYFGTFDGRLIALDAKTGKLVWSVNTIPPEAELGKQRSYTV 198 + CCD +NRG A + V+FGT D +L+AL+ TGK+VWS + Y++ Sbjct: 129 --RPCCDVINRGVALYGNLVFFGTLDAKLVALNKDTGKVVWSKKVAD------HKEGYSI 180 Query: 199 DGAPRIAKGRVIIGNGGSEFGARGFVSAFDAETGKVDWRFFTVPNPKN----EPDAASDS 254 AP I G++I G G EFG G + A++ E G++ W TV + A + Sbjct: 181 SAAPMIVNGKLITGVAGGEFGVVGKIQAYNPENGELLWMRPTVEGHMGYVYKDGKAIENG 240 Query: 255 VLMNKAYQTWSPTGAWTRQGGGGTVWDSIVYDPVADLVYLGVGNGSPWNYKYRSEGKGDN 314 + +A +TW P W + GG W YDP +L+ G GN +PWN R GDN Sbjct: 241 ISGGEAGKTW-PGDLW--KTGGAAPWLGGYYDPETNLILFGTGNPAPWNSHLRP---GDN 294 Query: 315 LFLGSIVALKPETGEYVWHFQETPMDQWDFTSDQQIMTLDLPINGETRHVIVHARKNGFF 374 L+ S +AL P+ G WHFQ TP D WDF ++++ + G+ A +NGFF Sbjct: 295 LYSSSRLALNPDDGTIKWHFQSTPHDGWDFDGVNELISFNYKDGGKEVKAAATADRNGFF 354 Query: 375 YIIDAKTGEFISGKNYV-YVNWASGLDPKTGRPIYNPDALYTLTGKEWYG-----IPGDL 428 Y++D G+FI G +V + WA+GLD K GRPIYN + G E G P L Sbjct: 355 YVLDRTNGKFIRGFPFVDKITWATGLD-KDGRPIYNDASRPGAPGSEAKGSSVFVAPAFL 413 Query: 429 GGHNFAAMAFSPKTGLVYIPAQQVPFLYTNQVGGFTPHPDSWNLGLDMNK-VGIPDSPEA 487 G N+ MA++ TGL Y+P+ N+ G D WN G+ K + Sbjct: 414 GAKNWMPMAYNKDTGLFYVPS--------NEWG-----MDIWNEGIAYKKGAAFLGAGFT 460 Query: 488 KQAFVKDLKGWIVAWDPQKQAEAWRVDHKGPWNGGILATGGDLLFQGLANGEFHAYDATN 547 + +D G + A DP E WR + P GG+L T G+L+F G G A++A Sbjct: 461 IKPLNEDYIGVLRAIDPVSGKEVWRHKNYAPLWGGVLTTKGNLVFTGTPEGFLQAFNAKT 520 Query: 548 GSDLFHFAADSGIIAPPVTYLANGKQYVAVEVGWGGIYPFFLGGLAR 594 G ++ F SG++ PVT+ +G+QYV+V GWGG P + G +A+ Sbjct: 521 GDKVWEFQTGSGVLGSPVTWEMDGEQYVSVVSGWGGAVPLWGGEVAK 567 Lambda K H 0.318 0.137 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1809 Number of extensions: 166 Number of successful extensions: 17 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 738 Length of database: 595 Length adjustment: 38 Effective length of query: 700 Effective length of database: 557 Effective search space: 389900 Effective search space used: 389900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory