Aligments for a candidate for dopDH in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Aligments for a candidate for dopDH in Pseudomonas putida KT2440

Align 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized)
to candidate PP_3602 PP_3602 2,5-dioxovalerate dehydrogenase

Query= BRENDA::Q88GW5
         (526 letters)



>FitnessBrowser__Putida:PP_3602
          Length = 526

 Score = 1040 bits (2689), Expect = 0.0
 Identities = 526/526 (100%), Positives = 526/526 (100%)

Query: 1   MPEILGHNFIAGQRSAAGPQRLQSLDASTGEALPYSFAQATEAEVDKAAKAAAAAFADFR 60
           MPEILGHNFIAGQRSAAGPQRLQSLDASTGEALPYSFAQATEAEVDKAAKAAAAAFADFR
Sbjct: 1   MPEILGHNFIAGQRSAAGPQRLQSLDASTGEALPYSFAQATEAEVDKAAKAAAAAFADFR 60

Query: 61  QLAPARRAEFLDAIAAELDELDDAFVAIVCRETALPAARIQGERGRTSGQMRLFAQVLRR 120
           QLAPARRAEFLDAIAAELDELDDAFVAIVCRETALPAARIQGERGRTSGQMRLFAQVLRR
Sbjct: 61  QLAPARRAEFLDAIAAELDELDDAFVAIVCRETALPAARIQGERGRTSGQMRLFAQVLRR 120

Query: 121 GDFLGARIDLALPERQPLPRVDLRQMRIGVGPVAVFGASNFPLAFSTAGGDTAAALAAGC 180
           GDFLGARIDLALPERQPLPRVDLRQMRIGVGPVAVFGASNFPLAFSTAGGDTAAALAAGC
Sbjct: 121 GDFLGARIDLALPERQPLPRVDLRQMRIGVGPVAVFGASNFPLAFSTAGGDTAAALAAGC 180

Query: 181 PVVFKAHSGHMATADLVACAIVRAAERTGMPKGVFNMVFGGGVGEWLVKHPAIQAVGFTG 240
           PVVFKAHSGHMATADLVACAIVRAAERTGMPKGVFNMVFGGGVGEWLVKHPAIQAVGFTG
Sbjct: 181 PVVFKAHSGHMATADLVACAIVRAAERTGMPKGVFNMVFGGGVGEWLVKHPAIQAVGFTG 240

Query: 241 SLKGGDALCRMAAERPQPIPVFAEMSSINPVIILPGALAKRGEAIARELAGSVCMGAGQF 300
           SLKGGDALCRMAAERPQPIPVFAEMSSINPVIILPGALAKRGEAIARELAGSVCMGAGQF
Sbjct: 241 SLKGGDALCRMAAERPQPIPVFAEMSSINPVIILPGALAKRGEAIARELAGSVCMGAGQF 300

Query: 301 CTNPGLVIGLQSPQYSQLLADLGQYLDQQAGQTMLNAGGLHSYVGGLEHLHAHAGIEHVA 360
           CTNPGLVIGLQSPQYSQLLADLGQYLDQQAGQTMLNAGGLHSYVGGLEHLHAHAGIEHVA
Sbjct: 301 CTNPGLVIGLQSPQYSQLLADLGQYLDQQAGQTMLNAGGLHSYVGGLEHLHAHAGIEHVA 360

Query: 361 GQVQEGNQARAQLFKADARLLVESDPLLQEEVFGPTTVAVEVQDNDQLRDALLGLRGQLT 420
           GQVQEGNQARAQLFKADARLLVESDPLLQEEVFGPTTVAVEVQDNDQLRDALLGLRGQLT
Sbjct: 361 GQVQEGNQARAQLFKADARLLVESDPLLQEEVFGPTTVAVEVQDNDQLRDALLGLRGQLT 420

Query: 421 ATLIGEAEDFDAFAWLVPLLEEKVGRILVNGYPTGVEVCDAMVHGGPYPATSDARGTSVG 480
           ATLIGEAEDFDAFAWLVPLLEEKVGRILVNGYPTGVEVCDAMVHGGPYPATSDARGTSVG
Sbjct: 421 ATLIGEAEDFDAFAWLVPLLEEKVGRILVNGYPTGVEVCDAMVHGGPYPATSDARGTSVG 480

Query: 481 TLAIDRFLRPVCYQNYPQTLLPEALRDGNPLGLRRLVNGQWSDGAI 526
           TLAIDRFLRPVCYQNYPQTLLPEALRDGNPLGLRRLVNGQWSDGAI
Sbjct: 481 TLAIDRFLRPVCYQNYPQTLLPEALRDGNPLGLRRLVNGQWSDGAI 526


Lambda     K      H
   0.321    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1008
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 526
Length adjustment: 35
Effective length of query: 491
Effective length of database: 491
Effective search space:   241081
Effective search space used:   241081
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources