GapMind for catabolism of small carbon sources

 

Aligments for a candidate for braC in Pseudomonas putida KT2440

Align BraC, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC (characterized)
to candidate PP_4867 PP_4867 branched-chain amino acid ABC transporter, periplasmic amino acid-binding protein (braC-like)

Query= TCDB::Q9L3M3
         (381 letters)



>lcl|FitnessBrowser__Putida:PP_4867 PP_4867 branched-chain amino
           acid ABC transporter, periplasmic amino acid-binding
           protein (braC-like)
          Length = 378

 Score =  334 bits (856), Expect = 3e-96
 Identities = 180/363 (49%), Positives = 233/363 (64%), Gaps = 6/363 (1%)

Query: 2   KKSLLSAVALTAMLAFSGNAWADVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINAAGGI 61
           KK  L A+A+   L  S    ADV I VAGP+TG NAAFG Q  KGA+ AA  INAAGG+
Sbjct: 7   KKGFL-ALAVATALGVSSYVQADVKIGVAGPMTGANAAFGEQYMKGAQAAADKINAAGGV 65

Query: 62  NGEQIKIELGDDVSDPKQGISVANKFA-ADGVKFVIGHFNSGVSIPASEVYAENGILRNH 120
           NGE+I +  GDD  +PKQ ++VAN+    D V  V+GHF S  +IPASEVY E G++   
Sbjct: 66  NGEKIVLVKGDDACEPKQAVAVANRLVDQDKVIGVVGHFCSSNTIPASEVYDEAGVIAIT 125

Query: 121 PGRDEPDLHGTGLWNTFRTCGRDDQQGAIAGKYLADHFKDAKIAVVHDKTPYGQGLADET 180
           PG   P +   GL   FR CGRDDQQG +AG Y+ D  K  K+AV+HDK  YGQGLAD T
Sbjct: 126 PGSTNPQVTERGLSAMFRMCGRDDQQGIVAGDYIVDVLKGKKVAVLHDKDTYGQGLADAT 185

Query: 181 KKAMNAAGVTEVIYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQAADQGL 240
           K  +   GV  V+YEG+  G+KDFSA++ K++  G  ++Y+GGLH EAG ++RQ  +QGL
Sbjct: 186 KAQLEKRGVKPVLYEGLTRGEKDFSAVVTKIRSTGADVVYFGGLHPEAGPLVRQLREQGL 245

Query: 241 K-ATLVSGDGIVSNELASIAGDA--VAGTLNTFGPDPTANPANKELVEKFKAAGFNPEAY 297
           K    +S DGIV++EL S AG A  V G   TFG DP   P +K +VE+F+ AG  PE Y
Sbjct: 246 KDVKFMSDDGIVTDELVSTAGGAQYVDGVYMTFGADPRLLPDSKAVVEEFRKAGTEPEGY 305

Query: 298 TLYSYAAMQTIAGAAKAAGSLDPEAVAKAMKEKGPFPTVLGDISFDEKGDPKIPGYIMYE 357
           TLY+YA++Q +A A   A S   E  AK +K   P  TV+G+  +D KGD  +  Y++Y+
Sbjct: 306 TLYAYASLQALAAAFNGAKSNKGEDAAKWLK-ANPVQTVMGEKKWDSKGDLTVSDYVVYQ 364

Query: 358 WKK 360
           W K
Sbjct: 365 WDK 367


Lambda     K      H
   0.314    0.132    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 456
Number of extensions: 24
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 381
Length of database: 378
Length adjustment: 30
Effective length of query: 351
Effective length of database: 348
Effective search space:   122148
Effective search space used:   122148
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory