Align Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized)
to candidate PP_4749 PP_4749 Amino acid ABC transporter, permease protein
Query= TCDB::Q9I404 (222 letters) >FitnessBrowser__Putida:PP_4749 Length = 219 Score = 120 bits (301), Expect = 2e-32 Identities = 74/210 (35%), Positives = 116/210 (55%), Gaps = 7/210 (3%) Query: 11 LPSLWEGMLMTLKLMVLGVLGGVALGTVLALMRLSHSKLLSNIAGFYVNYFRSIPLLLVI 70 LP LW+G ++++++ + + GV LG +LAL S S+ L + V R P L+++ Sbjct: 12 LPELWKGFVLSMQVTAVSLGLGVVLGLLLALGVSSPSRWLRYPSLVVVELGRGAPALILL 71 Query: 71 TWFYFAVPFILRWITGEDTPVGAFTSCLVAFMMFEAAYYCEIVRAGIQAIPKGQMGAAQA 130 +FYF +P + +T +F + +A AAY EI+R GI+++ GQ AA+ Sbjct: 72 QYFYFGLPSVNLTLT-------SFWAAALAMAYCTAAYTSEIIRGGIESVAAGQTEAAEV 124 Query: 131 LGMTYGQTMRLVILPQAFRKMTPLLLQQSIILFQDTSLVYTVGLMDFLNSARSRGDIIGQ 190 +G+ +R VILPQA R P LL SI++FQ +SL +T+ L + ++ A S G + Sbjct: 125 IGLNRFDALRFVILPQALRVALPSLLGFSIMMFQASSLCFTIALPEIVSRASSIGSSTFE 184 Query: 191 ANEFLIFAGLVYFVVSFTASFAVKRLQKRL 220 LI AGL+Y V A+ V L+KRL Sbjct: 185 YMPVLILAGLMYAAVCAPATLGVAALEKRL 214 Lambda K H 0.331 0.143 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 135 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 222 Length of database: 219 Length adjustment: 22 Effective length of query: 200 Effective length of database: 197 Effective search space: 39400 Effective search space used: 39400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory