GapMind for catabolism of small carbon sources

 

Alignments for a candidate for glpT in Pseudomonas putida KT2440

Align GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized)
to candidate PP_2261 PP_2261 Sugar ABC transporter, ATP-binding protein

Query= TCDB::G3LHY9
         (356 letters)



>FitnessBrowser__Putida:PP_2261
          Length = 365

 Score =  392 bits (1007), Expect = e-114
 Identities = 200/358 (55%), Positives = 251/358 (70%), Gaps = 11/358 (3%)

Query: 1   MARITLDHIRHAYGANPKSDKDYSLKEVDHEWNDGGAYALLGPSGCGKTTLLNIISGLLQ 60
           MA I L  + H+Y   P S+ DY+L  ++H W  GGAYALLGPSGCGK+TLLNIISGLL 
Sbjct: 1   MAEIRLRQLAHSYSRQPASEADYALHALEHVWEQGGAYALLGPSGCGKSTLLNIISGLLT 60

Query: 61  PSHGRILFDGKDVTNLSTQSRNIAQVFQFPVIYDTMTVYDNLAFPLRNRGVAEADVDRRV 120
           PSHG +LFDGK V  LS Q+RNIAQVFQFPV+YDTMTV+DNLAFPLRN+G+ EA V  RV
Sbjct: 61  PSHGEVLFDGKPVNQLSPQARNIAQVFQFPVVYDTMTVFDNLAFPLRNQGLDEARVRARV 120

Query: 121 RDILEMIDLASWARRKAQGLTADQKQKISLGRGLVRNDVNAILFDEPLTVIDPHMKWVLR 180
            +I E+++L +  R+KA+ LTAD+KQK+S+GRGLVR+DV+AILFDEPLTVIDPH+KW LR
Sbjct: 121 EEIAEVLELTAVLRKKARNLTADEKQKVSMGRGLVRDDVSAILFDEPLTVIDPHLKWKLR 180

Query: 181 SQLKRLHKQFGFTMVYVTHDQTEALTFAEKVVVMYDGQIVQIGTPAELFERPSHTFVGYF 240
            +LK++H+QF  TM+YVTHDQ EA TFA+K+ VM+ G+IVQ GTP ELFERP HTFVGYF
Sbjct: 181 RKLKQIHEQFNITMIYVTHDQLEASTFADKIAVMHGGRIVQFGTPRELFERPRHTFVGYF 240

Query: 241 IGSPGMNFMPARIEGSTVKVGDETLTL-----EYAPKTSGTAKTELGIRPEFIRLG---- 291
           IGSPGMN +  R E   V  GD  L L     E     +G  + ++GIRPEF++L     
Sbjct: 241 IGSPGMNLIDVRAEAGAVVFGDVQLVLPDGLCERLASLAG-GRLQVGIRPEFVQLWDSPF 299

Query: 292 REGMPITISKVEDIGRQKIVRARFADQPIAIVVPEDADIPADAR-VTFDPSAISIYAD 348
               P  +  VED+G  +IV        +   + ED  +P D   V+     + +Y D
Sbjct: 300 EGAYPARVLDVEDLGTYRIVTLELGGVALKARLGEDRPLPVDQTWVSLPAQWLMLYVD 357


Lambda     K      H
   0.321    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 430
Number of extensions: 17
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 365
Length adjustment: 29
Effective length of query: 327
Effective length of database: 336
Effective search space:   109872
Effective search space used:   109872
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory